Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis

Pallavi Sinha; Pradyot Prakash; Shashikant C.U. Patne; Shampa Anupurba; Sweety Gupta; G. N. Srivastava

doi:10.1007/s12275-017-6127-y

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Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis: Pallavi Sinha ¹, Pradyot Prakash ¹, Shashikant C.U. Patne ², Shampa Anupurba ¹, Sweety Gupta ¹, G. N. Srivastava ³; Journal of Microbiology 2017;55(1):63-67
DOI: https://doi.org/10.1007/s12275-017-6127-y
Published online: December 30, 2016

¹Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India, ²Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India, ³Department of Respiratory Diseases, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India¹Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India, ²Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India, ³Department of Respiratory Diseases, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India

Received: 15 March 2016 • Revised: 29 July 2016 • Accepted: 28 September 2016

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Abstract

The conventional methods for diagnosis of tubercular lymphadenitis (TBLN) such as - fine needle aspiration cytology, Ziehl-Neelsen staining and culture have limitations of low sensitivity and/or specificity. So, it becomes essential to develop a rapid, sensitive, and specific method for an early diagnosis of TBLN. Therefore, the present study was conducted to evaluate nested multiplex polymerase chain reaction (nMPCR) targeting MTP40 and IS6110 gene sequences of Mycobacterium tuberculosis and Mycobacterium tuberculosis complex, respectively in 48 successive patients of TBLN and 20 random patients with non-tubercular lymph node lesions. Out of the 48 cases of TBLN, 14 (29.2%) were found to be positive by Ziehl-Neelsen staining, 15 (31.2%) were positive by culture and 43 (89.6%) cases were positive after first round of PCR while 48 (100%) cases were positive by nMPCR assay. The sensitivity and specificity of nMPCR was found to be 100% for the diagnosis of TBLN. The results thus obtained indicate that nMPCR assay is a highly sensitive and specific tool for the diagnosis of TBLN.

Keywords: fine needle aspiration; Mycobacterium tuberculosis; nested multiplex PCR; Ziehl-Neelsen staining

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Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis

J. Microbiol. 2017;55(1):63-67. Published online December 30, 2016

DOI: https://doi.org/10.1007/s12275-017-6127-y

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