Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1

Sung Ho Yun; Sang-Yeop Lee; Chi-Won Choi; Hayoung Lee; Hyun-Joo Ro; Sangmi Jun; Yong Min Kwon; Kae Kyoung Kwon; Sang-Jin Kim; Gun-Hwa Kim; Seung Il Kim

doi:10.1007/s12275-017-6581-6

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Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1: Sung Ho Yun ¹, Sang-Yeop Lee ¹, Chi-Won Choi ², Hayoung Lee ³, Hyun-Joo Ro ¹, Sangmi Jun ¹, Yong Min Kwon ⁴, Kae Kyoung Kwon ⁵, Sang-Jin Kim ^4,5, Gun-Hwa Kim ¹, Seung Il Kim ^1,6; Journal of Microbiology 2017;55(1):56-62
DOI: https://doi.org/10.1007/s12275-017-6581-6
Published online: December 30, 2016

¹Drug and Disease Target Team, Korea Basic Science Institute, Daejeon 34133, Republic of Korea, ²Tunneling Nanotube Research Center, Division of Life Science, Korea University, Seoul 02841, Republic of Korea, ³Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea, ⁴National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea, ⁵Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea, ⁶National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea, 5Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea¹Drug and Disease Target Team, Korea Basic Science Institute, Daejeon 34133, Republic of Korea, ²Tunneling Nanotube Research Center, Division of Life Science, Korea University, Seoul 02841, Republic of Korea, ³Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea, ⁴National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea, ⁵Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea, ⁶National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea, 5Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea

Received: 14 November 2016 • Revised: 6 December 2016 • Accepted: 8 December 2016

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Abstract

Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25–70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane- associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.

Keywords: Novosphingobium pentaromativorans; outer membrane vesicle; proteomics

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Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1

J. Microbiol. 2017;55(1):56-62. Published online December 30, 2016

DOI: https://doi.org/10.1007/s12275-017-6581-6

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