Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1

Sung Ho Yun; Sang-Yeop Lee; Chi-Won Choi; Hayoung Lee; Hyun-Joo Ro; Sangmi Jun; Yong Min Kwon; Kae Kyoung Kwon; Sang-Jin Kim; Gun-Hwa Kim; Seung Il Kim

doi:10.1007/s12275-017-6581-6

Articles

Page Path: HOME > J. Microbiol > Volume 55(1); 2017 > Article

Article
Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1: Sung Ho Yun ¹, Sang-Yeop Lee ¹, Chi-Won Choi ², Hayoung Lee ³, Hyun-Joo Ro ¹, Sangmi Jun ¹, Yong Min Kwon ⁴, Kae Kyoung Kwon ⁵, Sang-Jin Kim ^4,5, Gun-Hwa Kim ¹, Seung Il Kim ^1,6; Journal of Microbiology 2017;55(1):56-62.
DOI: https://doi.org/10.1007/s12275-017-6581-6
Published online: December 30, 2016

¹Drug and Disease Target Team, Korea Basic Science Institute, Daejeon 34133, Republic of Korea, ²Tunneling Nanotube Research Center, Division of Life Science, Korea University, Seoul 02841, Republic of Korea, ³Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon 34113, Republic of Korea, ⁴National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea, ⁵Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea, ⁶National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea, 5Korea Institute of Ocean Science and Technology, Ansan 15627, Republic of Korea

Received: 14 November 2016 • Revised: 6 December 2016 • Accepted: 8 December 2016

prev next

575 Views
0 Download
18 Crossref
21 Scopus

Article

Download PDF

Abstract

Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25–70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane- associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.

Keywords: Novosphingobium pentaromativorans;outer membrane vesicle;proteomics

Supplementary Information

References

Citations

Citations to this article as recorded by

Multi-omic analysis reveals the microecological mechanism of organic arsenic toxicity in a Japanese abandoned chemical weapons site and targeted bioremediation strategies
Xu YANG, Xiao-hui JI, Chen LI, Jin-long LAI, Xue-gang LUO
Journal of Hazardous Materials.2026; 503: 141101. CrossRef
Bacterial extracellular vesicles: Vital contributors to physiology from bacteria to host
Xinke Nie, Qiqiong Li, Xinyang Chen, Stanley Onyango, Junhua Xie, Shaoping Nie
Microbiological Research.2024; 284: 127733. CrossRef
Marine Delivery Vehicles: Molecular Components and Applications of Bacterial Extracellular Vesicles
Angela Casillo, Raffaele D’Amico, Rosa Lanzetta, Maria Michela Corsaro
Marine Drugs.2024; 22(8): 363. CrossRef
Impact of probiotics-derived extracellular vesicles on livestock gut barrier function
Yuhan Zhang, Mengzhen Song, Jinping Fan, Xuming Guo, Shiyu Tao
Journal of Animal Science and Biotechnology.2024;[Epub] CrossRef
Genomic and physiological characterization of Novosphingobium terrae sp. nov., an alphaproteobacterium isolated from Cerrado soil containing a mega-sized chromid
Aline Belmok, Felipe Marques de Almeida, Rodrigo Theodoro Rocha, Carla Simone Vizzotto, Marcos Rogério Tótola, Marcelo Henrique Soller Ramada, Ricardo Henrique Krüger, Cynthia Maria Kyaw, Georgios J. Pappas
Brazilian Journal of Microbiology.2023; 54(1): 239. CrossRef
Proteomic and Functional Analyses of Outer Membrane Vesicles Secreted by Vibrio splendidus
Huimin Song, Yilong Ruan, Ya Li, Huirong Yang, Weiwei Zhang
Journal of Ocean University of China.2023; 22(5): 1361. CrossRef
Bacterial membrane vesicle functions, laboratory methods, and applications
Pınar Aytar Çelik, Burak Derkuş, Kübra Erdoğan, Dilan Barut, Enuh Blaise Manga, Yalın Yıldırım, Simon Pecha, Ahmet Çabuk
Biotechnology Advances.2022; 54: 107869. CrossRef
The relationship between bacterial outer membrane vesicles and halophilic adaptation
Dilan Barut, Blaise M Enuh, Burak Derkuş, Ülkü Güler, Bekir Salih, Pınar Aytar Çelik
Molecular Omics.2022; 19(2): 174. CrossRef
Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes
Steven J. Biller, Rachel A. Lundeen, Laura R. Hmelo, Kevin W. Becker, Aldo A. Arellano, Keven Dooley, Katherine R. Heal, Laura T. Carlson, Benjamin A. S. Van Mooy, Anitra E. Ingalls, Sallie W. Chisholm
Environmental Microbiology.2022; 24(1): 420. CrossRef
Benzo[a]pyrene might be transported by a TonB-dependent transporter in Novosphingobium pentaromativorans US6-1
Jiaqing Liang, Jiantao Xu, Weijun Zhao, Jiaofeng Wang, Kai Chen, Yuqian Li, Yun Tian
Journal of Hazardous Materials.2021; 404: 124037. CrossRef
Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692
Silindile Maphosa, Lucy Novungayo Moleleki
Microorganisms.2021; 9(9): 1918. CrossRef
Eco-evolutionary feedbacks mediated by bacterial membrane vesicles
Nikola Zlatkov, Aftab Nadeem, Bernt Eric Uhlin, Sun Nyunt Wai
FEMS Microbiology Reviews.2021;[Epub] CrossRef
Analysis of the Extracellular Proteome of Colistin-Resistant Korean Acinetobacter baumannii Strains
Sang-Yeop Lee, Sung Ho Yun, Hayoung Lee, Yoon-Sun Yi, Edmond Changkyun Park, Wooyoung Kim, Hye-Yeon Kim, Je Chul Lee, Gun-Hwa Kim, Seung Il Kim
ACS Omega.2020; 5(11): 5713. CrossRef
Comprehensive proteomic analysis and pathogenic role of membrane vesicles of Listeria monocytogenes serotype 4b reveals proteins associated with virulence and their possible interaction with host
Raman Karthikeyan, Pratapa Gayathri, Paramasamy Gunasekaran, Medicharla V. Jagannadham, Jeyaprakash Rajendhran
International Journal of Medical Microbiology.2019; 309(3-4): 199. CrossRef
Novosphingobium sp. PP1Y as a novel source of outer membrane vesicles
Federica De Lise, Francesca Mensitieri, Giulia Rusciano, Fabrizio Dal Piaz, Giovanni Forte, Flaviana Di Lorenzo, Antonio Molinaro, Armando Zarrelli, Valeria Romanucci, Valeria Cafaro, Antonio Sasso, Amelia Filippelli, Alberto Di Donato, Viviana Izzo
Journal of Microbiology.2019; 57(6): 498. CrossRef
Proteomic and Metabolomic Analyses of Xylella fastidiosa OMV-Enriched Fractions Reveal Association with Virulence Factors and Signaling Molecules of the DSF Family
Oséias R. Feitosa-Junior, Eliezer Stefanello, Paulo A. Zaini, Rafael Nascimento, Paulo M. Pierry, Abhaya M. Dandekar, Steven E. Lindow, Aline M. da Silva
Phytopathology®.2019; 109(8): 1344. CrossRef
Biophysical restriction of growth area using a monodispersed gold sphere nanobarrier prolongs the mitotic phase in HeLa cells
Dae-Woong Jung, Hyun-Joo Ro, Junmin Kim, Seung Il Kim, Gi-Ra Yi, Gaehang Lee, Sangmi Jun
RSC Advances.2019; 9(64): 37497. CrossRef
Extracellular membrane vesicles in the three domains of life and beyond
Sukhvinder Gill, Ryan Catchpole, Patrick Forterre
FEMS Microbiology Reviews.2019; 43(3): 273. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1

J. Microbiol. 2017;55(1):56-62. Published online December 30, 2016

DOI: https://doi.org/10.1007/s12275-017-6581-6

Related articles