Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima

Rina Hidayati Pratiwi; Iman Hidayat; Muhammad Hanafi; Wibowo Mangunwardoyo

doi:10.1007/s12275-017-6311-0

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Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima: Rina Hidayati Pratiwi ^1,2, Iman Hidayat ³, Muhammad Hanafi ⁴, Wibowo Mangunwardoyo ²; Journal of Microbiology 2017;55(4):289-295.
DOI: https://doi.org/10.1007/s12275-017-6311-0
Published online: January 26, 2017

¹Department of Biological Education, Faculty of Technics, Mathematics and Natural Sciences, University of Indraprasta PGRI, South Jakarta 12530, Indonesia, ²Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424, Indonesia, ³Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia, ⁴Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), PUSPIPTEK, Serpong 15314, Indonesia¹Department of Biological Education, Faculty of Technics, Mathematics and Natural Sciences, University of Indraprasta PGRI, South Jakarta 12530, Indonesia, ²Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424, Indonesia, ³Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Cibinong 16911, Indonesia, ⁴Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), PUSPIPTEK, Serpong 15314, Indonesia

Corresponding author: Rina Hidayati Pratiwi , Tel: rina.hp2012@gmail.com,

Received: 30 June 2016 • Revised: 15 December 2016 • Accepted: 20 December 2016

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Abstract

This study’s aim was to determine the identity of antibacte-rial compounds produced by Pseudomonas aeruginosa strain UICC B-40 and describe the antibacterial compounds’ me-chanisms of action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carried out using thin layer chromatography (TLC), nuclear mag-netic resonance (NMR) spectroscopy and liquid chromato-graphy mass spectrometry (LC-MS) analyses. Antibacterial activity was assayed via minimum inhibitory concentration (MIC) and the antibacterial compound mechanism was ob-served morphologically through scanning electron micros-copy (SEM). This study successfully identified the (2E,5E)- phenyltetradeca-2,5-dienoate antibacterial compound (mole-cular weight 300 g/mol), composed of a phenolic ester, fatty acid and long chain of aliphatic group structures. MIC values for this compound were determined at 62.5 μg/ml against Staphylococcus aureus strain ATCC 25923. The mechanism of the compound involved breaking down the bacterial cell walls through the lysis process. The (2E,5E)-phenyltetradeca- 2,5-dienoate compound exhibited inhibitory activity on the growth of Gram-positive bacteria.

Keywords: antibacterial;bioactive;endophytic bacteria;Neesia altissima;Pseudomonas aeruginosa strain UICC B-40

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Antibacterial compound produced by Pseudomonas aeruginosa strain UICC B-40, an endophytic bacterium isolated from Neesia altissima

J. Microbiol. 2017;55(4):289-295. Published online January 26, 2017

DOI: https://doi.org/10.1007/s12275-017-6311-0

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