Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet

YingWu Shi; Chun Li Li; HongMei Yang; Tao Zhang; Yan Gao; Min Chu; Jun Zeng; Qing Lin; OuTiKu Er; YuGuo Li; Xiangdong Huo; Kai Lou

doi:10.1007/s12275-017-6371-1

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Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet: YingWu Shi ^1,2, Chun Li Li ^1,2, HongMei Yang ^1,2, Tao Zhang ^1,2, Yan Gao ^1,2, Min Chu ^1,2, Jun Zeng ^1,2, Qing Lin ^1,2, OuTiKu Er ^1,2, YuGuo Li ^1,2, Xiangdong Huo ^1,2, Kai Lou ^1,2; Journal of Microbiology 2017;55(4):267-272.
DOI: https://doi.org/10.1007/s12275-017-6371-1
Published online: January 26, 2017

¹Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China, ²Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China¹Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, P. R. China, ²Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, Xinjiang, P. R. China

Corresponding author: Kai Lou , Tel: +86-991-4521590,

Received: 9 August 2016 • Revised: 25 October 2016 • Accepted: 2 November 2016

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Abstract

This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its coloniza-tion of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed us-ing fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inocu-lated plants, and the survival of the Achromobacter marpla-tensis 17:gfp strain in plants 20 days after inoculation, even in the absence of selective pressure, suggests that it is good colonizer. These results also suggest that this strain could be a useful tool for the delivery of enzymes or other proteins into plants. In addition, the study highlights that sugar beet plants can be used effectively for detailed in vitro studies on the interactions between A. marplatensis strain 17 and its host, particularly if a gfp-tagged strain of the pathogen is used.

Keywords: sugar beet;endophytes;green fluorescent pro-tein;colonization

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Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet

J. Microbiol. 2017;55(4):267-272. Published online January 26, 2017

DOI: https://doi.org/10.1007/s12275-017-6371-1

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