Rapid MALDI biotyper-based identification and cluster analysis of Streptococcus iniae

Si Won Kim; Seong Won Nho; Se Pyeong Im; Jung Seok Lee; Jae Wook Jung; Jassy Mary S. Lazarte; Jaesung Kim; Woo-Jai Lee; Jeong-Ho Lee; Tae Sung Jung

doi:10.1007/s12275-017-6472-x

Articles

Page Path: HOME > J. Microbiol > Volume 55(4); 2017 > Article

Journal Article
Rapid MALDI biotyper-based identification and cluster analysis of Streptococcus iniae: Si Won Kim ¹, Seong Won Nho ², Se Pyeong Im ¹, Jung Seok Lee ¹, Jae Wook Jung ¹, Jassy Mary S. Lazarte ¹, Jaesung Kim ¹, Woo-Jai Lee ³, Jeong-Ho Lee ⁴, Tae Sung Jung ¹; Journal of Microbiology 2017;55(4):260-266.
DOI: https://doi.org/10.1007/s12275-017-6472-x
Published online: January 26, 2017

¹Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea, ²Division of Microbiology, National Center for Toxicological Research/FDA, Jefferson, Arkansas, USA, ³BluGen Korea, Busan 48071, Republic of Korea, ⁴Inland Aquaculture Research Center, NIFS, Changwon 51695, Republic of Korea¹Laboratory of Aquatic Animal Diseases, Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea, ²Division of Microbiology, National Center for Toxicological Research/FDA, Jefferson, Arkansas, USA, ³BluGen Korea, Busan 48071, Republic of Korea, ⁴Inland Aquaculture Research Center, NIFS, Changwon 51695, Republic of Korea

Corresponding author: Tae Sung Jung , Tel: +82-55-751-5822,

Received: 21 September 2016 • Revised: 2 December 2016 • Accepted: 15 December 2016

prev next

46 Views
0 Download
8 Crossref
10 Scopus

Full Article

Abstract

Streptococcus iniae causes severe mortalities among cultured marine species, especially in the olive flounder (Paralichthys olivaceus), which is economically important in Korea and Japan. Recently, there has been growing concern regarding the emergence of S. iniae as a zoonotic pathogen. Here, 89 S. iniae isolates obtained from diseased olive flounders collected from 2003 to 2008 in Jeju Island, South Korea, were charac-terized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The results were aligned both with the available Bruker Daltonics data-base and with a new set of S. iniae data entries developed in our laboratory, and the results were compared. When we used the Bruker Daltonics database, the 89 isolates yielded either “no reliable identification” or were incorrectly iden-tified as Streptococcus pyogenes at the genus level. When we used the new data entries from our laboratory, in contrast, all of the isolates were correctly identified as S. iniae at the genus (100%) and species (96.6%) levels. We performed pro-teomic analysis, divided the 89 isolates into cluster I (51.7%), cluster II (20.2%), and cluster III (28.1%), and then used the MALDI Biotyper software to identify specific mass peaks that enabled discrimination between clusters and between Strep-tococcus species. Our results suggest that the use of MALDI TOF MS could outperform the conventional methods, prov-ing easier, faster, cheaper and more efficient in properly identifying S. iniae. This strategy could facilitate the epide-miological and taxonomical study of this important fish pathogen.

Keywords: MALDI TOF MS;Streptococcus iniae;olive floun-der;Paralichthys olivaceus;database

Figure & Data

References

Citations

Citations to this article as recorded by

Identification of Vagococcus salmoninarum from rainbow trout (Oncorhynchus mykiss Walbaum, 1792) using proteomics–based MALDI–TOF MS
İfakat Tülay Çağatay
Revista Científica de la Facultad de Ciencias Veterinarias.2024; XXXIV(2): 1. CrossRef
Automatic Identification of MALDI-TOF MS Database Using Classical Bordetella Species Isolates
Yamin Liu, Junwen Cui, Chunhua Qie, Bei Jiang, Ying Li, Xiaoyun Zhao, Xue Fei Deng
Computational and Mathematical Methods in Medicine.2022; 2022: 1. CrossRef
Development of a real‐time PCR assay for detection and quantification of Streptococcus iniae using the lactate permease gene
Yolanda Torres‐Corral, Ysabel Santos
Journal of Fish Diseases.2021; 44(1): 53. CrossRef
Identification of bacterial pathogens in cultured fish with a custom peptide database constructed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Patharapol Piamsomboon, Janthima Jaresitthikunchai, Tran Quang Hung, Sittiruk Roytrakul, Janenuj Wongtavatchai
BMC Veterinary Research.2020;[Epub] CrossRef
An improved method for MALDI-TOF analysis of wine-associated yeasts
Junwen Zhang, Jeffrey E. Plowman, Bin Tian, Stefan Clerens, Stephen L.W. On
Journal of Microbiological Methods.2020; 172: 105904. CrossRef
Identification and typing of Vagococcus salmoninarum using genomic and proteomic techniques
Yolanda Torres‐Corral, Ysabel Santos
Journal of Fish Diseases.2019; 42(4): 597. CrossRef
Proteomic and molecular fingerprinting for identification and tracking of fish pathogenic Streptococcus
Torres-Corral Yolanda, Fernández-Álvarez Clara, Santos Ysabel
Aquaculture.2019; 498: 322. CrossRef
Use of MALDI-TOF Mass Spectrometry for the Fast Identification of Gram-Positive Fish Pathogens
Gabriella B. N. Assis, Felipe L. Pereira, Alexandra U. Zegarra, Guilherme C. Tavares, Carlos A. Leal, Henrique C. P. Figueiredo
Frontiers in Microbiology.2017;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Rapid MALDI biotyper-based identification and cluster analysis of Streptococcus iniae

J. Microbiol. 2017;55(4):260-266. Published online January 26, 2017

DOI: https://doi.org/10.1007/s12275-017-6472-x

Related articles