Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps

Hongli Gong; Boyan Wang; Yi Shi; Yong Shi; Xiyan Xiao; Pengyu Cao; Lei Tao; Yuezhu Wang; Liang Zhou

doi:10.1007/s12275-017-6636-8

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Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps: Hongli Gong ¹, Boyan Wang ², Yi Shi ³, Yong Shi ¹, Xiyan Xiao ¹, Pengyu Cao ¹, Lei Tao ¹, Yuezhu Wang ⁴, Liang Zhou ¹; Journal of Microbiology 2017;55(8):648-654.
DOI: https://doi.org/10.1007/s12275-017-6636-8
Published online: July 28, 2017

¹Shanghai Key Clinical Disciplines of Otorhinolaryngology, Department Otorhinolaryngology, Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai, 200031, P. R. China, ²Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China, ³Department of Clinical Laboratory, Branch of Shanghai First People's Hospital, Shanghai, 200081, and Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, P. R. China, ⁴Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Sequencing Center, Shanghai, 201203, P. R. China¹Shanghai Key Clinical Disciplines of Otorhinolaryngology, Department Otorhinolaryngology, Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai, 200031, P. R. China, ²Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China, ³Department of Clinical Laboratory, Branch of Shanghai First People's Hospital, Shanghai, 200081, and Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, P. R. China, ⁴Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Sequencing Center, Shanghai, 201203, P. R. China

Corresponding author: Yi Shi , Tel: +86-136-2182-0215,
Liang Zhou , Tel: +86-136-2182-0215,

Received: 13 December 2016 • Revised: 13 July 2017 • Accepted: 13 July 2017

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Abstract

The pharynx is an important site of microbiota colonization, but the bacterial populations at this site have been relatively unexplored by culture-independent approaches. The aim of this study was to characterize the microbiota structure of the pharynx. Pyrosequencing of 16S rRNA gene libraries was used to characterize the pharyngeal microbiota using swab samples from 68 subjects with laryngeal cancer and 28 subjects with vocal cord polyps. Overall, the major phylum was Firmicutes, with Streptococcus as the predominant genus in the pharyngeal communities. Nine core operational taxonomic units detected from Streptococcus, Fusobacterium, Prevotella, Granulicatella, and Veillonella accounted for 21.3% of the total sequences detected. However, there was no difference in bacterial communities in the pharynx from patients with laryngeal cancer and vocal cord polyps. The relative abundance of Firmicutes was inversely correlated with Fusobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes. The correlation was evident at the genus level, and the relative abundance of Streptococcus was inversely associated with Fusobacterium, Leptotrichia, Neisseria, Actinomyces, and Prevotella. This study presented a profile for the overall structure of the microbiota in pharyngeal swab samples. Inverse correlations were found between Streptococcus and other bacterial communities, suggesting that potential antagonism may exist among pharyngeal microbiota.

Keywords: microbiota;bacterial communities;pharynx;laryngeal cancer;vocal cord polyps

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Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps

J. Microbiol. 2017;55(8):648-654. Published online July 28, 2017

DOI: https://doi.org/10.1007/s12275-017-6636-8

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