A rapid and simple method for identifying bacterial polar lipid components in wet biomass

Tuan Manh Nguyen; Jaisoo Kim

doi:10.1007/s12275-017-7092-1

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A rapid and simple method for identifying bacterial polar lipid components in wet biomass: Tuan Manh Nguyen ^1,2, Jaisoo Kim ¹; Journal of Microbiology 2017;55(8):635-639.
DOI: https://doi.org/10.1007/s12275-017-7092-1
Published online: July 4, 2017

¹Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Republic of Korea, ²Thai Nguyen University of Agriculture and Forestry, Quyet Thang Commune, Thai Nguyen City, Vietnam¹Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Republic of Korea, ²Thai Nguyen University of Agriculture and Forestry, Quyet Thang Commune, Thai Nguyen City, Vietnam

Corresponding author: Jaisoo Kim , Tel: +82-31-249-9648,

Received: 28 February 2017 • Revised: 17 May 2017 • Accepted: 2 June 2017

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Abstract

There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach. Moreover, our improved method ensures simple lipid extraction. Overall, the results of the analysis showed minor lipid profile differences between the two approaches with regard to quantity, and lipid identification was consistent in both methods for all species.

Keywords: polar lipid;wet cells;freeze-dried cells;species classification;lipid extraction;chemotaxonomy

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A rapid and simple method for identifying bacterial polar lipid components in wet biomass

J. Microbiol. 2017;55(8):635-639. Published online July 4, 2017

DOI: https://doi.org/10.1007/s12275-017-7092-1

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