The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants

Ki Bum Ahn; A Reum Kim; Kee-Yeon Kum; Cheol-Heui Yun; Seung Hyun Han

doi:10.1007/s12275-017-7362-y

Articles

Page Path: HOME > J. Microbiol > Volume 55(10); 2017 > Article

Journal Article
The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants: Ki Bum Ahn ^1,2, A Reum Kim ¹, Kee-Yeon Kum ³, Cheol-Heui Yun ⁴, Seung Hyun Han ⁵; Journal of Microbiology 2017;55(10):830-836.
DOI: https://doi.org/10.1007/s12275-017-7362-y
Published online: September 28, 2017

¹Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, ²Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ³Department of Conservative Dentistry and DRI, School of Dentistry,Seoul National University, Seoul 03080, Republic of Korea, ⁴Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, ⁵1Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea¹Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, ²Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ³Department of Conservative Dentistry and DRI, School of Dentistry,Seoul National University, Seoul 03080, Republic of Korea, ⁴Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, ⁵1Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea

Corresponding author: Seung Hyun Han , Tel: +82-2-880-2310,

Received: 23 August 2017 • Accepted: 3 September 2017

46 Views
0 Download
26 Crossref
34 Scopus

Full Article

Abstract

Streptococcus mutans is a major etiologic agent of human dental caries that forms biofilms on hard tissues in the human oral cavity, such as tooth and dentinal surfaces. Human β-defensin-3 (HBD3) is a 45-amino-acid natural antimicrobial peptide that has broad spectrum antimicrobial activity against bacteria and fungi. A synthetic peptide consisting of the C-terminal 15 amino acids of HBD3 (HBD3-C15) was recently shown to be sufficient for its antimicrobial activity. Thus, clinical applications of this peptide have garnered attention. In this study, we investigated whether HBD3-C15 inhibits the growth of the representative cariogenic pathogen Streptococcus mutans and its biofilm formation. HBD3-C15 inhibited bacterial growth, exhibited bactericidal activity, and attenuated bacterial biofilm formation in a dose-dependent manner. HBD3-C15 potentiated the bactericidal and anti-biofilm activity of calcium hydroxide (CH) and chlorhexidine digluconate (CHX), which are representative disinfectants used in dental clinics, against S. mutans. Moreover, HBD3-C15 showed antimicrobial activity by inhibiting biofilm formation by S. mutans and other dentinophilic bacteria such as Enterococcus faecalis and Streptococcus gordonii, which are associated with dental caries and endodontic infection, on human dentin slices. These effects were observed for HBD3-C15 alone and for HBD3-C15 in combination with CH or CHX. Therefore, we suggest that HBD3-C15 is a potential alternative or additive disinfectant that can be used for the treatment of oral infectious diseases, including dental caries and endodontic infections.

Keywords: Streptococcus mutans;HBD3;biofilm;antimicrobial activity;disinfectant

Figure & Data

References

Citations

Citations to this article as recorded by

Human β-defensins and their synthetic analogs: Natural defenders and prospective new drugs of oral health
Mumian Chen, Zihe Hu, Jue Shi, Zhijian Xie
Life Sciences.2024; 346: 122591. CrossRef
Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment
Sara Takallu, Esmaeil Mirzaei, Abbas Zakeri Bazmandeh, Hamid Reza Ghaderi Jafarbeigloo, Hooman Khorshidi
ACS Infectious Diseases.2024; 10(3): 779. CrossRef
Novel Nano Drug‐Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI
Jibin Wu, Jianling Yan, Sijia Xu, Xuan Zou, Yinghua Xu, Xiaobao Jin, Xuemei Lu, Shuiqing Gui
Advanced Healthcare Materials.2024;[Epub] CrossRef
Harnessing antimicrobial peptides in endodontics
Xinzi Kong, Vijetha Vishwanath, Prasanna Neelakantan, Zhou Ye
International Endodontic Journal.2024; 57(7): 815. CrossRef
A Robust Strategy Against Multi-Resistant Pathogens in Oral Health: Harnessing the Potency of Antimicrobial Peptides in Nanofiber-Mediated Therapies
Karthikeyan Kandaswamy, Raghunandhakumar Subramanian, Jayant Giri, Ajay Guru, Jesu Arockiaraj
International Journal of Peptide Research and Therapeutics.2024;[Epub] CrossRef
A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action
Athira P. P., Anju M. V., Anooja V. V., Archana K., Neelima S., Muhammed Musthafa S., I. S. Bright Singh, Rosamma Philip
Marine Biotechnology.2024; 26(4): 696. CrossRef
Antimicrobial and Antibiofilm Activity of Synthetic Peptide [W7]KR12-KAEK Against Enterococcus faecalis Strains
Mayron Alves Vasconcelos, Bruno Rocha da Silva, Alexandre Lopes Andrade, Aryane de Azevedo Pinheiro, Francisco Flávio Vasconcelos Evaristo, Francisco Vassiliepe Sousa Arruda, Esteban Nicolás Lorenzón, Eduardo Maffud Cilli, Edson Holanda Teixeira
Current Microbiology.2023;[Epub] CrossRef
Peptide Designs for Use in Caries Management: A Systematic Review
Olivia Lili Zhang, John Yun Niu, Ollie Yiru Yu, May Lei Mei, Nicholas Stephen Jakubovics, Chun Hung Chu
International Journal of Molecular Sciences.2023; 24(4): 4247. CrossRef
Growing Global Research Interest in Antimicrobial Peptides for Caries Management: A Bibliometric Analysis
Olivia Lili Zhang, John Yun Niu, Iris Xiaoxue Yin, Ollie Yiru Yu, May Lei Mei, Chun Hung Chu
Journal of Functional Biomaterials.2022; 13(4): 210. CrossRef
Important Roles and Potential Uses of Natural and Synthetic Antimicrobial Peptides (AMPs) in Oral Diseases: Cavity, Periodontal Disease, and Thrush
Albert Donald Luong, Alyah Buzid, John H. T. Luong
Journal of Functional Biomaterials.2022; 13(4): 175. CrossRef
Development and evaluation of a chewing gum containing antimicrobial peptide GH12 for caries prevention
Xuelian Jiang, Yufei Wang, Xinwei Li, Zening Feng, Yuhao Zeng, Sili Han, Nobuhiro Takahashi, Linglin Zhang
European Journal of Oral Sciences.2022;[Epub] CrossRef
Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from Enterococcus faecium DB1 on Cariogenic Streptococcus mutans Biofilm Formation
Ni-Na Kim, Bong Sun Kim, Han Bin Lee, Sunghyun An, Donghan Kim, Seok-Seong Kang
Food Science of Animal Resources.2022; 42(6): 1020. CrossRef
In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae
Yankang Wu, Na Yang, Ruoyu Mao, Ya Hao, Da Teng, Jianhua Wang
Microorganisms.2022; 10(5): 881. CrossRef
Characterization of Histopathology and Microbiota in Contemporary Regenerative Endodontic Procedures: Still Coming up Short
Yeon-Jee Yoo, Hiran Perinpanayagam, Youngnim Choi, Yu Gu, Seok-Woo Chang, Seung-Ho Baek, Qiang Zhu, Ashraf F. Fouad, Kee-Yeon Kum
Journal of Endodontics.2021; 47(8): 1285. CrossRef
The multifaceted roles of antimicrobial peptides in oral diseases
John Yun Niu, Iris Xiaoxue Yin, May Lei Mei, William Ka Kei Wu, Quan‐Li Li, Chun Hung Chu
Molecular Oral Microbiology.2021; 36(3): 159. CrossRef
Antimicrobial peptides: mechanism of action, activity and clinical potential
Qi-Yu Zhang, Zhi-Bin Yan, Yue-Ming Meng, Xiang-Yu Hong, Gang Shao, Jun-Jie Ma, Xu-Rui Cheng, Jun Liu, Jian Kang, Cai-Yun Fu
Military Medical Research.2021;[Epub] CrossRef
Antimicrobial peptides for the prevention and treatment of dental caries: A concise review
John Yun Niu, Iris Xiaoxue Yin, William Ka Kei Wu, Quan-Li Li, May Lei Mei, Chun Hung Chu
Archives of Oral Biology.2021; 122: 105022. CrossRef
Suppressive effects of gecko cathelicidin on biofilm formation and cariogenic virulence factors of Streptococcus mutans
Shasha Cai, Kai Meng, Peng Liu, Xianting Cao, Guannan Wang
Archives of Oral Biology.2021; 129: 105205. CrossRef
A Chimeric Cationic Peptide Composed of Human β-Defensin 3 and Human β-Defensin 4 Exhibits Improved Antibacterial Activity and Salt Resistance
Wenjing Yu, Nianzhi Ning, Ying Xue, Yanyu Huang, Feng Guo, Tao Li, Boning Yang, Deyan Luo, Yakun Sun, Zhan Li, Jianxin Wang, Zhili He, Shiwei Cheng, Xingxiao Zhang, Hui Wang
Frontiers in Microbiology.2021;[Epub] CrossRef
Oral antimicrobial peptides and new therapeutic strategies for plaque-mediated diseases
Nafiseh Izadi, Masoud Keikha, Kiarash Ghazvini, Mohsen Karbalaei
Gene Reports.2020; 21: 100811. CrossRef
Antimicrobial peptide GH12 as root canal irrigant inhibits biofilm and virulence of Enterococcus faecalis
Y. Li, Y. Wang, X. Chen, W. Jiang, X. Jiang, Y. Zeng, X. Li, Z. Feng, J. Luo, L. Zhang
International Endodontic Journal.2020; 53(7): 948. CrossRef
Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans
Kaixin Xiong, Xuan Chen, Hantao Hu, Huihui Hou, Peng Gao, Ling Zou
BioMed Research International.2020; 2020: 1. CrossRef
In Vitro Evaluation of the Antibacterial Properties of Tea Tree Oil on Planktonic and Biofilm-Forming Streptococcus mutans
Yu-Meng Song, Hong-Yan Zhou, Ye Wu, Jue Wang, Qian Liu, Yu-Feng Mei
AAPS PharmSciTech.2020;[Epub] CrossRef
Endodontic biofilms: contemporary and future treatment options
Yeon-Jee Yoo, Hiran Perinpanayagam, Soram Oh, A-Reum Kim, Seung-Hyun Han, Kee-Yeon Kum
Restorative Dentistry & Endodontics.2019;[Epub] CrossRef
Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats
Yufei Wang, Yuhao Zeng, Yuanjing Wang, Haoran Li, Sihan Yu, Wentao Jiang, Yingxue Li, Linglin Zhang
Journal of Oral Microbiology.2019; 11(1): 1549921. CrossRef
A New Combination with D-Cateslytin to Eradicate Root Canal Pathogens
Claire Ehlinger, Pauline Dartevelle, Abdurraouf Zaet, Yoshihito Kurashige, Youssef Haïkel, Marie-Hélène Metz-Boutigue, Céline Marban
International Journal of Peptide Research and Therapeutics.2019; 25(4): 1679. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants

J. Microbiol. 2017;55(10):830-836. Published online September 28, 2017

DOI: https://doi.org/10.1007/s12275-017-7362-y

Related articles