Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo

Prasanna Weeratunga; Thilina U. B. Herath; Tae-Hwan Kim; Hyun-Cheol Lee; Jae-Hoon Kim; Byeong-Hoon Lee; Eun-Seo Lee; Kiramage Chathuranga; W. A. Gayan Chathuranga; Chul-Su Yang; Jin Yeul Ma; Jong-Soo Lee

doi:10.1007/s12275-017-7392-5

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Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo: Prasanna Weeratunga ¹, Thilina U. B. Herath ¹, Tae-Hwan Kim ¹, Hyun-Cheol Lee ¹, Jae-Hoon Kim ¹, Byeong-Hoon Lee ¹, Eun-Seo Lee ¹, Kiramage Chathuranga ¹, W. A. Gayan Chathuranga ¹, Chul-Su Yang ², Jin Yeul Ma ³, Jong-Soo Lee ¹; Journal of Microbiology 2017;55(11):909-917.
DOI: https://doi.org/10.1007/s12275-017-7392-5
Published online: October 27, 2017

¹College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea, ²Department of Molecular and Life Science, College of Science and Technology, Hanyang University, Ansan 15588, Republic of Korea, ³Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Republic of Korea¹College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea, ²Department of Molecular and Life Science, College of Science and Technology, Hanyang University, Ansan 15588, Republic of Korea, ³Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Republic of Korea

Corresponding author: Jong-Soo Lee , Tel: +82-42-821-6753,

Received: 14 September 2017 • Revised: 15 October 2017 • Accepted: 16 October 2017

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Abstract

Dense granule protein-7 (GRA-7) is an excretory protein of Toxoplasma gondii. It is a potential serodiagnostic marker and vaccine candidate for toxoplasmosis. Previous reports demonstrated that GRA-7 induces innate immune responses in macrophages by interacting with TRAF6 via the MyD88- dependent pathway. In the present study, we evaluated the antiviral activity and induction of an antiviral state by GRA-7 both in vitro and in vivo. It was observed that GRA-7 markedly reduced the replication of vesicular stomatitis virus (VSVGFP), influenza A virus (PR8-GFP), coxsackievirus (H3- GFP), herpes simplex virus (HSV-GFP), and adenovirus-GFP in epithelial (HEK293T/HeLa) and immune (RAW264.7) cells. These antiviral activities of GRA-7 were attributed to the induction of type I interferon (IFN) signaling, resulting in the secretion of IFNs and pro-inflammatory cytokines. Additionally, in BALB/c mice, intranasal administration of GRA-7 prevented lethal infection by influenza A virus (H1N1) and exhibited prophylactic effects against respiratory syncytial virus (RSV-GFP). Collectively, these results suggested that GRA-7 exhibits immunostimulatory and broad spectrum antiviral activities via type I IFN signaling. Thus, GRA-7 can be potentially used as a vaccine adjuvant or as a candidate drug with prophylactic potential against viruses.

Keywords: Toxoplasma gondii;GRA-7;antiviral activity

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Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo

J. Microbiol. 2017;55(11):909-917. Published online October 27, 2017

DOI: https://doi.org/10.1007/s12275-017-7392-5

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