Recombinant baculovirus-based vaccine expressing M2 protein induces protective CD8+ T-cell immunity against respiratory syncytial virus infection

Jeong-Yoon Lee; Jun Chang

doi:10.1007/s12275-017-7306-6

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Recombinant baculovirus-based vaccine expressing M2 protein induces protective CD8+ T-cell immunity against respiratory syncytial virus infection: Jeong-Yoon Lee , Jun Chang; Journal of Microbiology 2017;55(11):900-908.
DOI: https://doi.org/10.1007/s12275-017-7306-6
Published online: October 27, 2017

Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Republic of KoreaGraduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Republic of Korea

Corresponding author: Jun Chang , Tel: +82-2-3277-2549,

Received: 28 July 2017 • Revised: 10 September 2017 • Accepted: 11 October 2017

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Abstract

Respiratory syncytial virus (RSV) is an important cause of acute lower respiratory tract disease in infants, young children, immunocompromised individuals, and the elderly. However, despite ongoing efforts to develop an RSV vaccine, there is still no authorized RSV vaccine for humans. Baculovirus has attracted attention as a vaccine vector because of its ability to induce a high level of humoral and cellular immunity, low cytotoxicity against various antigens, and biological safety for humans. In this study, we constructed a recombinant baculovirus- based vaccine expressing the M2 protein of RSV under the control of cytomegalovirus promoter (Bac_RSVM2) to induce CD8+ T-cell responses which play an important role in viral clearance, and investigated its protective efficacy against RSV infection. Immunization with Bac_RSVM2 via intranasal or intramuscular route effectively elicited the specific CD8+ T-cell responses. Most notably, immunization with Bac_RSVM2 vaccine almost completely protected mice from RSV challenge without vaccine-enhanced immunopathology. In conclusion, these results suggest that Bac_RSVM2 vaccine employing the baculovirus delivery platform has promising potential to be developed as a safe and novel RSV vaccine that provides protection against RSV infection.

Keywords: recombinant baculovirus;respiratory syncytial virus;m2 protein;cd8 t cells;vaccine

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Recombinant baculovirus-based vaccine expressing M2 protein induces protective CD8+ T-cell immunity against respiratory syncytial virus infection

J. Microbiol. 2017;55(11):900-908. Published online October 27, 2017

DOI: https://doi.org/10.1007/s12275-017-7306-6

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