The NADPH oxidase AoNoxA in Arthrobotrys oligospora functions as an initial factor in the infection of Caenorhabditis elegans

Xin Li; Ying-Qian Kang; Yan-Lu Luo; Ke-Qin Zhang; Cheng-Gang Zou; Lian-Ming Liang

doi:10.1007/s12275-017-7169-x

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The NADPH oxidase AoNoxA in Arthrobotrys oligospora functions as an initial factor in the infection of Caenorhabditis elegans: Xin Li ^1,2, Ying-Qian Kang ³, Yan-Lu Luo ¹, Ke-Qin Zhang ¹, Cheng-Gang Zou ¹, Lian-Ming Liang ¹; Journal of Microbiology 2017;55(11):885-891.
DOI: https://doi.org/10.1007/s12275-017-7169-x
Published online: October 27, 2017

¹State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan 650091, P. R. China, ²Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan 650500, P. R. China, ³Department of Microbiology, Guizhou Medical University, Guiyang, Guizhou 550025, P. R. China¹State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan 650091, P. R. China, ²Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan 650500, P. R. China, ³Department of Microbiology, Guizhou Medical University, Guiyang, Guizhou 550025, P. R. China

Corresponding author: Cheng-Gang Zou , Tel: +86-871-65034838,
Lian-Ming Liang , Tel: +86-871-65034838,

Received: 19 April 2017 • Revised: 6 September 2017 • Accepted: 26 September 2017

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Abstract

Reactive oxygen species (ROS) produced by NADPH oxidases can serve as signaling molecules to regulate a variety of physiological processes in multi-cellular organisms. In the nematophagous fungus Arthrobotrys oligospora, we found that ROS were produced during conidial germination, hyphal extension, and trap formation in the presence of nematodes. Generation of an AoNoxA knockout strain demonstrated the crucial role of NADPH oxidase in the production of ROS in A. oligospora, with trap formation impaired in the AoNoxA mutant, even in the presence of the nematode host. In addition, the expression of virulence factor serine protease P186 was up-regulated in the wild-type strain, but not in the mutant strain, in the presence of Caenorhabditis elegans. These results indicate that ROS derived from AoNoxA are essential for full virulence of A. oligospora in nematodes.

Keywords: NADPH oxidase;nematode-trapping fungi;trap formation;reactive oxygen species

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The NADPH oxidase AoNoxA in Arthrobotrys oligospora functions as an initial factor in the infection of Caenorhabditis elegans

J. Microbiol. 2017;55(11):885-891. Published online October 27, 2017

DOI: https://doi.org/10.1007/s12275-017-7169-x

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