Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica

Yong Heon Lee; Ji Hye Kim

doi:10.1007/s12275-017-7410-7

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Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica: Yong Heon Lee ^1,2, Ji Hye Kim ¹; Journal of Microbiology 2017;55(12):966-972.
DOI: https://doi.org/10.1007/s12275-017-7410-7
Published online: December 7, 2017

¹School of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea, ²Department of Biomedical Laboratory Science, Dongseo University, Busan 47011, Republic of Korea¹School of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea, ²Department of Biomedical Laboratory Science, Dongseo University, Busan 47011, Republic of Korea

Received: 27 September 2017 • Revised: 18 October 2017 • Accepted: 23 October 2017

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Abstract

In Salmonella enterica serovar Typhimurium, the acid-sensing regulator CadC activates transcription of the cadBA operon which contributes to the acid tolerance response. The DNA-binding response regulator OmpR in two-component regulatory system with EnvZ binds to its own promoter for autoinduction. We previously reported that CadC exerts a negative influence on ompR transcription during acid adaptation. However, its underlying mechanisms remain to be elucidated. Here we show that the level of OmpR protein is gradually reduced by a gradual increase in the CadC level using an arabinose-inducible expression system, indicating there exists a negative correlation between the expression levels of two transcription factors. To explore the molecular basis for OmpR repression by CadC, we performed in vitro binding assays and determined that CadC directly interacts with OmpR. We further show that inactivation of cadC inhibits transcription of the fliC gene, which encodes the major flagellar subunit,
result
ing in impaired flagellar motility under acid-adaptation conditions. Together, our findings suggest that CadC may repress autoinduction of the OmpR response regulator through their direct interaction.

Keywords: Salmonella enterica;CadC;OmpR;fliC;acid stress

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Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica

J. Microbiol. 2017;55(12):966-972. Published online December 7, 2017

DOI: https://doi.org/10.1007/s12275-017-7410-7

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