The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus

Mercedeh Tajbakhsh; Abdollah Karimi; Abolghasem Tohidpour; Naser Abbasi; Fatemeh Fallah; Maziar Mohammad Akhavan

doi:10.1007/s12275-018-7444-5

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The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus: Mercedeh Tajbakhsh ¹, Abdollah Karimi ¹, Abolghasem Tohidpour ², Naser Abbasi ^3,4, Fatemeh Fallah ¹, Maziar Mohammad Akhavan ^5,6; Journal of Microbiology 2018;56(2):128-137.
DOI: https://doi.org/10.1007/s12275-018-7444-5
Published online: February 2, 2018

¹Pediatric Infections Research Center (PIRC), Research Institute for Children health, Shahid Beheshti University of Medical Sciences, Tehran, Iran, ²Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University, Krasnoyarsk, Russia, ³Department of Pharmacology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran, ⁴Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran, ⁵Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, ⁶Department of Pharmacology, Medical School, Iran University of Medical Sciences, Tehran, Iran¹Pediatric Infections Research Center (PIRC), Research Institute for Children health, Shahid Beheshti University of Medical Sciences, Tehran, Iran, ²Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University, Krasnoyarsk, Russia, ³Department of Pharmacology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran, ⁴Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran, ⁵Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, ⁶Department of Pharmacology, Medical School, Iran University of Medical Sciences, Tehran, Iran

Corresponding author: Maziar Mohammad Akhavan , Tel: +98-2122741-507,

Received: 18 October 2017 • Revised: 28 November 2017 • Accepted: 11 December 2017

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Abstract

Cathelicidins are a family of antimicrobial peptides which exhibit broad antimicrobial activities against antibiotic-resistant bacteria. Considering the progressive antibiotic resistance, cathelicidin is a candidate for use as an alternative approach to treat and overcome the challenge of antimicrobial resistance. Cathelicidin-BF (Cath-BF) is a short antimicrobial peptide, which was originally extracted from the venom of Bungarus fasciatus. Recent studies have reported that Cath-BF and some related derivatives exert strong antimicrobial and weak hemolytic properties. This study investigates the bactericidal and cytotoxic effects of Cath-BF and its analogs (Cath-A and Cath-B). Cath-A and Cath-B were designed to increase their net positive charge, to have more activity against methicillin resistant S. aureus (MRSA). The results of this study show that Cath-A, with a +17-net charge, has the most noteworthy antimicrobial activity against MRSA strains, with minimum inhibitory concentration (MIC) ranging between 32–128 μg/ml. The bacterial kinetic analysis by 1 × MIC concentration of each peptide shows that Cath-A neutralizes the clinical MRSA isolate for 60 min. The present data support the notion that increasing the positive net charge of antimicrobial peptides can increase their potential antimicrobial activity. Cath-A also displayed the weakest cytotoxicity effect against human umbilical vein endothelial and H9c2 rat cardiomyoblast cell lines. Analysis of the hemolytic activity reveals that all three peptides exhibit minor hemolytic activity against human erythrocytes at concentrations up to 250 μg/ml. Altogether, these results suggest that Cath-A and Cath-B are competent candidates as novel antimicrobial compounds against MRSA and possibly other multidrug resistant bacteria.

Keywords: antibiotic resistance;antimicrobial peptides;Cathelicidin- BF;cytotoxic activity;MRSA

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The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus

J. Microbiol. 2018;56(2):128-137. Published online February 2, 2018

DOI: https://doi.org/10.1007/s12275-018-7444-5

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