Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine

Min Yong Jwa; Soyoung Jeong; Eun Byeol Ko; A Reum Kim; Hyun Young Kim; Sun Kyung Kim; Ho Seong Seo; Cheol-Heui Yun; Seung Hyun Han

doi:10.1007/s12275-018-8347-1

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Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine: Min Yong Jwa ¹, Soyoung Jeong ¹, Eun Byeol Ko ¹, A Reum Kim ¹, Hyun Young Kim ¹, Sun Kyung Kim ¹, Ho Seong Seo ², Cheol-Heui Yun ³, Seung Hyun Han ¹; Journal of Microbiology 2018;56(8):579-585.
DOI: https://doi.org/10.1007/s12275-018-8347-1
Published online: July 25, 2018

¹Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, ²Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ³Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea¹Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, ²Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, ³Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

Corresponding author: Seung Hyun Han , Tel: +82-2-880-2310;,

Received: 26 June 2018 • Revised: 2 July 2018 • Accepted: 2 July 2018

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Abstract

Streptococcus pneumoniae is a major respiratory pathogen that causes millions of deaths worldwide. Although subunit vaccines formulated with the capsular polysaccharides or their protein conjugates are currently-available, low-cost vaccines with wide serotype coverage still remain to be developed, especially for developing countries. Recently, gamma- irradiation has been considered as an effective inactivation
method
to prepare S. pneumoniae vaccine candidate. In this study, we investigated the immunogenicity and protective immunity of gamma-irradiated S. pneumoniae (r-SP), by comparing with heat-inactivated S. pneumoniae (h-SP) and formalin-inactivated S. pneumoniae (f-SP), both of which were made by traditional inactivation methods. Intranasal immunization of C57BL/6 mice with r-SP in combination with cholera toxin as an adjuvant enhanced S. pneumoniaespecific antibodies on the airway mucosal surface and in sera more potently than that with h-SP or f-SP under the same conditions. In addition, sera from mice immunized with r- SP potently induced opsonophagocytic killing activity more effectively than those of h-SP or f-SP, implying that r-SP could induce protective antibodies. Above all, immunization with r-SP effectively protected mice against S. pneumoniae infection. Collectively, these results suggest that gamma- irradiation is an effective method for the development of a killed whole cell pneumococcal vaccine that elicits robust mucosal and systemic immune responses.

Keywords: vaccine;Streptococcus pneumoniae;gamma-irradiation;killed whole cell vaccine

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Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine

J. Microbiol. 2018;56(8):579-585. Published online July 25, 2018

DOI: https://doi.org/10.1007/s12275-018-8347-1

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