[PROTOCOL] Drosophila melanogaster as a polymicrobial infection model for Pseudomonas aeruginosa and Staphylococcus aureus

Young-Joon Lee; Hye-Jeong Jang; In-Young Chung; You-Hee Cho

doi:10.1007/s12275-018-8331-9

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[PROTOCOL] Drosophila melanogaster as a polymicrobial infection model for Pseudomonas aeruginosa and Staphylococcus aureus: Young-Joon Lee , Hye-Jeong Jang , In-Young Chung , You-Hee Cho; Journal of Microbiology 2018;56(8):534-541.
DOI: https://doi.org/10.1007/s12275-018-8331-9
Published online: July 25, 2018

Department of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, Republic of Korea

Corresponding author: You-Hee Cho , Tel: +82-31-881-7165,

Received: 18 June 2018 • Revised: 12 July 2018 • Accepted: 16 July 2018

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Abstract

Non-mammalian infection models have been developed over the last two decades, which is a historic milestone to understand the molecular basis of bacterial pathogenesis. They also provide small-scale research platforms for identification of virulence factors, screening for antibacterial hits, and evaluation of antibacterial efficacy. The fruit fly, Drosophila melanogaster is one of the model hosts for a variety of bacterial pathogens, in that the innate immunity pathways and tissue physiology are highly similar to those in mammals. We here present a relatively simple protocol to assess the key aspects of the polymicrobial interaction in vivo between the human opportunistic pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, which is based on the systemic infection by needle pricking at the dorsal thorax of the flies. After infection, fly survival and bacteremia over time for both P. aeruginosa and S. aureus within the infected flies can be monitored as a measure of polymicrobial virulence potential. The infection takes ~24 h including bacterial cultivation. Fly survival and bacteremia are assessed using the infected flies that are monitored up to ~60 h post-infection. These methods can be used to identify presumable as well as unexpected phenotypes during polymicrobial interaction between P. aeruginosa and S. aureus mutants, regarding bacterial pathogenesis and host immunity.

Keywords: Drosophila melanogaster;Pseudomonas aeruginosa;Staphylococcus aureus;polymicrobial infection;nonmammalian host

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[PROTOCOL] Drosophila melanogaster as a polymicrobial infection model for Pseudomonas aeruginosa and Staphylococcus aureus

J. Microbiol. 2018;56(8):534-541. Published online July 25, 2018

DOI: https://doi.org/10.1007/s12275-018-8331-9

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