Identification of trehalose as a compatible solute in different species of acidophilic bacteria

Pedro A. Galleguillos; Barry M. Grail; Kevin B. Hallberg; Cecilia S. Demergasso; D. Barrie Johnson

doi:10.1007/s12275-018-8176-2

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Identification of trehalose as a compatible solute in different species of acidophilic bacteria: Pedro A. Galleguillos ^1,2, Barry M. Grail ³, Kevin B. Hallberg ³, Cecilia S. Demergasso ^1,2, D. Barrie Johnson ³; Journal of Microbiology 2018;56(10):727-733.
DOI: https://doi.org/10.1007/s12275-018-8176-2
Published online: September 28, 2018

¹Scientific and Technological Research Centre for Mining (CICITEM), Sucre 220, Of. 602, Antofagasta, Chile, ²Biotechnology Centre, Catholic University of the North, Avda. Angamos 0610, Antofagasta, Chile, ³College of Natural Sciences, Bangor University, Bangor LL57 2UW, UK¹Scientific and Technological Research Centre for Mining (CICITEM), Sucre 220, Of. 602, Antofagasta, Chile, ²Biotechnology Centre, Catholic University of the North, Avda. Angamos 0610, Antofagasta, Chile, ³College of Natural Sciences, Bangor University, Bangor LL57 2UW, UK

Corresponding author: Pedro A. Galleguillos , Tel: +56-55-2651718,

Received: 30 March 2018 • Revised: 5 June 2018 • Accepted: 25 June 2018

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Abstract

The major industrial heap bioleaching processes are located in desert regions (mainly Chile and Australia) where fresh water is scarce and the use of resources with low water activity becomes an attractive alternative. However, in spite of the importance of the microbial populations involved in these processes, little is known about their response or adaptation to osmotic stress. In order to investigate the response to osmotic stress in these microorganisms, six species of acidophilic bacteria were grown at elevated osmotic strength in liquid media, and the compatible solutes synthesised were identified using ion chromatography and MALDI-TOF mass spectrometry. Trehalose was identified as one of, or the sole, compatible solute in all species and strains, apart from Acidithiobacillus thiooxidans where glucose and proline levels increased at elevated osmotic potentials. Several other potential compatible solutes were tentatively identified by MALDITOF analysis. The same compatible solutes were produced by these bacteria regardless of the salt used to produce the osmotic stress. The results correlate with data from sequenced genomes which confirm that many chemolithotrophic and heterotrophic acidophiles possess genes for trehalose synthesis. This is the first report to identify and quantify compatible solutes in acidophilic bacteria that have important roles in biomining technologies.

Keywords: bioleaching;acidophiles;compatible solutes;trehalose;sulfate;osmolytes

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Identification of trehalose as a compatible solute in different species of acidophilic bacteria

J. Microbiol. 2018;56(10):727-733. Published online September 28, 2018

DOI: https://doi.org/10.1007/s12275-018-8176-2

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