Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau

Hui-bin Lu; Xiao-feng Xue; Dorji Phurbu; Peng Xing; Qing-long Wu

doi:10.1007/s12275-018-8178-0

Articles

Page Path: HOME > J. Microbiol > Volume 56(11); 2018 > Article

Journal Article
Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau: Hui-bin Lu ^1,2, Xiao-feng Xue ³, Dorji Phurbu ⁴, Peng Xing ¹, Qing-long Wu ^1,5; Journal of Microbiology 2018;56(11):783-789.
DOI: https://doi.org/10.1007/s12275-018-8178-0
Published online: October 24, 2018

¹State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China, ²University of Chinese Academy of Sciences, Beijing 100049, P. R. China, ³Nanjing Normal University, Nanjing 210046, P. R. China, ⁴Tibet Plateau Institute of Biology, Lhasa 850000, P. R. China, ⁵Sino-Danish Centre for Education and Research, University of Chinese¹State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China, ²University of Chinese Academy of Sciences, Beijing 100049, P. R. China, ³Nanjing Normal University, Nanjing 210046, P. R. China, ⁴Tibet Plateau Institute of Biology, Lhasa 850000, P. R. China, ⁵Sino-Danish Centre for Education and Research, University of Chinese

Corresponding author: Peng Xing , Tel: +86-25-86882112,

Received: 2 April 2018 • Revised: 4 July 2018 • Accepted: 13 July 2018

prev next

47 Views
0 Download
6 Crossref
6 Scopus

Full Article

Abstract

Two Gram-stain negative halophilic strains, designated as LM2T and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase- positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2T and LM4 belong to the genus Roseovarius, with Roseovarius tolerans EL-172T (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084T (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were C18:1 ω7c/C18:1 ω6c, C16:0, C19:0 cyclo ω8c, and 11-methyl C18:1 ω7c. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2T and LM4, 24.9% relatedness between LM2T and R. tolerans EL-172T, and 36.3% relatedness between LM4 and R. tolerans EL-172T. Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2T and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius. It was evident that LM2T and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2T (= CGMCC 1.16230T = KCTC 62028T).

Keywords: Roseovarius;saline lake;LongmuCo;polyphasic taxonomy;Tibetan Plateau

Figure & Data

References

Citations

Citations to this article as recorded by

Lithoautotrophic lifestyle of the widespread genusRoseovariusrevealed by physiological and genomic characterization ofRoseovarius autotrophicussp. nov
Galina Slobodkina, Nataliya Ratnikova, Alexander Merkel, Vadim Kevbrin, Alexandra Kuchierskaya, Alexander Slobodkin
FEMS Microbiology Ecology.2022;[Epub] CrossRef
Duganella rivus sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella
Hui-Bin Lu, Zhi-Peng Cai, Yong-Gang Yang, Mei-Ying Xu
Antonie van Leeuwenhoek.2020; 113(8): 1155. CrossRef
Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China
Hui-Bin Lu, Tong-Chu Deng, Mei-Ying Xu
Current Microbiology.2020; 77(10): 3185. CrossRef
Halomonas rituensis sp. nov. and Halomonas zhuhanensis sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau
Peixin Gao, Huibin Lu, Peng Xing, Qinglong L. Wu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(10): 5217. CrossRef
Roseovarius spongiae sp. nov., a bacterium isolated from marine sponge
Lingping Zhuang, Lianzhong Luo
International Journal of Systematic and Evolutionary Microbiology .2020; 70(1): 274. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology.2019; 69(3): 597. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau

J. Microbiol. 2018;56(11):783-789. Published online October 24, 2018

DOI: https://doi.org/10.1007/s12275-018-8178-0

Related articles