Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection

In Young Choi; Cheonghoon Lee; Won Keun Song; Sung Jae Jang; Mi-Kyung Park

doi:10.1007/s12275-019-8610-0

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Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection: In Young Choi ¹, Cheonghoon Lee ^2,3, Won Keun Song ¹, Sung Jae Jang ², Mi-Kyung Park ^1,4; Journal of Microbiology 2019;57(2):170-179.
DOI: https://doi.org/10.1007/s12275-019-8610-0
Published online: January 31, 2019

¹School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, ²Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul 08826, Republic of Korea, ³Institute of Health and Environment, Seoul National University, Seoul 08826, Republic of Korea, ⁴Food and Bio-industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea¹School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, ²Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul 08826, Republic of Korea, ³Institute of Health and Environment, Seoul National University, Seoul 08826, Republic of Korea, ⁴Food and Bio-industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea

Corresponding author: Mi-Kyung Park , Tel: +82-53-950-5776,

Received: 8 November 2018 • Revised: 26 November 2018 • Accepted: 3 December 2018

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Abstract

Since Salmonella Enteritidis is one of the major foodborne pathogens, on-site applicable rapid detection methods have been required for its control. The purpose of this study was to isolate and purify S. Enteritidis-specific phage (KFS-SE2 phage) from an eel farm and to investigate its feasibility as a novel, efficient, and reliable bio-receptor for its employment. KFS-SE2 phage was successfully isolated at a high concentration of (2.31 ± 0.43) × 1011 PFU/ml, and consisted of an icosahedral head of 65.44 ± 10.08 nm with a non-contractile tail of 135.21 ± 12.41 nm. The morphological and phylogenetic analysis confirmed that it belongs to the Pis4avirus genus in the family of Siphoviridae. KFS-SE2 genome consisted of 48,608 bp with 45.7% of GC content. Genome analysis represented KFS-SE2 to have distinctive characteristics as a novel phage. Comparative analysis of KFS-SE2 phage with closely related strains confirmed its novelty by the presence of unique proteins. KFS-SE2 phage exhibited excellent specificity to S. Enteritidis and was stable under the temperature range of 4 to 50°C and pH of 3 to 11 (P < 0.05). The latent time was determined to be 20 min. Overall, a new lytic KFS-SE2 phage was successfully isolated from the environment at a high concentration and the excellent feasibility of KFS-SE2 phage was demonstrated as a new bio-receptor for S. Enteritidis detection.

Keywords: bio-receptor;phage;Salmonella Enteritidis;lytic;biosensor

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Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection

J. Microbiol. 2019;57(2):170-179. Published online January 31, 2019

DOI: https://doi.org/10.1007/s12275-019-8610-0

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