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REVIEW] Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
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REVIEW] Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
Inseong Jo 1, Jin-Sik Kim 2, Yongbin Xu 3, Jaekyung Hyun 4, Kangseok Lee 5, Nam-Chul Ha 1
Journal of Microbiology 2019;57(3):185-194
DOI: https://doi.org/10.1007/s12275-019-8520-1
Published online: February 26, 2019
1Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Center for Food and Bioconvergence, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, 2Unit on Structural and Chemical Biology of Membrane Proteins, Cell Biology and Neurobiology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA, 3Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian, 116600, Liaoning, P. R. China, 4Electron Microscopy Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea, 5Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea1Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Center for Food and Bioconvergence, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, 2Unit on Structural and Chemical Biology of Membrane Proteins, Cell Biology and Neurobiology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA, 3Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian, 116600, Liaoning, P. R. China, 4Electron Microscopy Research Center, Korea Basic Science Institute, Cheongju 28119, Republic of Korea, 5Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea
Corresponding author:  Nam-Chul Ha , Tel: +82-2-880-4853, 
Received: 20 September 2018   • Revised: 26 December 2018   • Accepted: 10 January 2019
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Tripartite efflux pumps and the type I secretion system of Gram-negative bacteria are large protein complexes that span the entire cell envelope. These complexes expel antibiotics and other toxic substances or transport protein toxins from bacterial cells. Elucidating the binary and ternary complex structures at an atomic resolution are crucial to understanding the assembly and working mechanism. Recent advances in cryoelectron microscopy along with the construction of chimeric proteins drastically shifted the assembly models. In this review, we describe the current assembly models from a historical perspective and emphasize the common assembly mechanism for the assembly of diverse tripartite pumps and type I secretion systems.

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    REVIEW] Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
    J. Microbiol. 2019;57(3):185-194.   Published online February 26, 2019
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