Role of putative virulence traits of Campylobacter jejuni in regulating differential host immune responses

Ankita Singh; Amirul Islam Mallick

doi:10.1007/s12275-019-8165-0

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Role of putative virulence traits of Campylobacter jejuni in regulating differential host immune responses: Ankita Singh , Amirul Islam Mallick; Journal of Microbiology 2019;57(4):298-309.
DOI: https://doi.org/10.1007/s12275-019-8165-0
Published online: February 22, 2019

Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, West Bengal, 741246, IndiaDepartment of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, West Bengal, 741246, India

Corresponding author: Amirul Islam Mallick , Tel: +91-33-2502-8000,

Received: 26 March 2018 • Revised: 16 October 2018 • Accepted: 24 October 2018

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Abstract

Among the major enteric pathogens, Campylobacter jejuni is considered an important source of diarrheal illness in humans. In contrast to the acute gastroenteritis in humans, C. jejuni exhibits prolonged cecal colonization at a high level with little or no pathology in chickens. Although several known virulence determinants of C. jejuni have been found to be associated with a higher degree of pathogenesis in humans, to date, little is known about their functions in the persistent colonization of chickens. The present study was undertaken to assess the role of C. jejuni in imparting differential host immune responses in human and chicken cells. Based on the abundance of major genes encoding virulence factors (GEVFs), we used a particular isolate that harbors the cadF, flaA, peb1, racR, ciaB, cdtB, and hcp genes. This study showed that hypervirulent C. jejuni isolate that encodes a functional type VI secretion system (T6SS) has a greater ability to invade and create characteristic “attaching and effacing” lesions in human INT407 compared to primary chicken embryo intestinal cells (CEICs). Furthermore, we demonstrated that the higher bacterial invasion in human INT407 triggered higher levels of expression of major proinflammatory cytokines, such as IL- 1β and IL-6, and significant downregulation of IL-17A gene expression (P ≤ 0.05). The findings of the present study suggest that the enhanced ability of C. jejuni to invade human cells is tightly regulated by proinflammatory cytokines in the gut and possibly holds the keys to the observed differences in pathogenesis between human and chicken cells.

Keywords: Campylobacter jejuni;chickens;cytokines;host response;virulence factors

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Role of putative virulence traits of Campylobacter jejuni in regulating differential host immune responses

J. Microbiol. 2019;57(4):298-309. Published online February 22, 2019

DOI: https://doi.org/10.1007/s12275-019-8165-0

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