Abstract
Aspergillus flavus is a saprophytic fungus that contaminates
crops with carcinogenic aflatoxin. In the present work, the
antifungal effects of volatile organic compounds (VOCs) from
Streptomyces alboflavus TD-1 against A. flavus were investigated.
VOCs from 8-day-old wheat bran culture of S. alboflavus
TD-1 displayed strong inhibitory effects against mycelial
growth, sporulation, and conidial germination of A.
flavus. Severely misshapen conidia and hyphae of A. flavus
were observed by scanning electron microscopy after exposure
to VOCs for 6 and 12 h, respectively. Rhodamine 123
staining of mitochondria indicated that mitochondria may
be a legitimate antifungal target of the VOCs from S. alboflavus
TD-1. Furthermore, the VOCs effectively inhibited
aflatoxin B1 production by downregulating genes involved
in aflatoxin biosynthesis. Dimethyl trisulfide and benzenamine
may play important roles in the suppression of A. flavus
growth and production of aflatoxin. The results indicate
that VOCs from S. alboflavus TD-1 have tremendous potential
to be developed as a useful bio-pesticide for controlling
A. flavus.
Citations
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