Transcriptome analysis to understand the effects of the toxoflavin and tropolone produced by phytopathogenic Burkholderia on Escherichia coli

Jungwook Park; Hyun-Hee Lee; Hyejung Jung; Young-Su Seo

doi:10.1007/s12275-019-9330-1

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Transcriptome analysis to understand the effects of the toxoflavin and tropolone produced by phytopathogenic Burkholderia on Escherichia coli: Jungwook Park ^1,2†, Hyun-Hee Lee ^1†, Hyejung Jung ^1†, Young-Su Seo ¹; Journal of Microbiology 2019;57(9):781-794.
DOI: https://doi.org/10.1007/s12275-019-9330-1
Published online: August 27, 2019

¹Department of Microbiology, Pusan National University, Busan 46241, Republic of Korea, ²Bioinformation Technology Division, Nakdonggang National Institute of Biological Resources (NNIBR), Sangju 37242, Republic of Korea¹Department of Microbiology, Pusan National University, Busan 46241, Republic of Korea, ²Bioinformation Technology Division, Nakdonggang National Institute of Biological Resources (NNIBR), Sangju 37242, Republic of Korea

Corresponding author: Young-Su Seo , Tel: +82-51-510-2267,

Received: 1 July 2019 • Revised: 18 July 2019 • Accepted: 25 July 2019

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Abstract

The phytopathogenic Burkholderia species B. glumae and B. plantarii are the causal agents of bacterial wilt, grain rot, and seedling blight, which threaten the rice industry globally. Toxoflavin and tropolone are produced by these phytopathogens and are considered the most hostile biohazards with a broad spectrum of target organisms. However, despite their nonspecific toxicity, the effects of toxoflavin and tropolone on bacteria remain unknown. RNA-seq based transcriptome analysis was employed to determine the genome-wide expression patterns under phytotoxin treatment. Expression of 2327 and 830 genes was differentially changed by toxoflavin and tropolone, respectively. Enriched biological pathways reflected the down-regulation of oxidative phosphorylation and ribosome function, beginning with the inhibition of membrane biosynthesis and nitrogen metabolism under oxidative stress or iron starvation. Conversely, several systems such as bacterial chemotaxis, flagellar assembly, biofilm formation, and sulfur/taurine transporters were highly expressed as countermeasures against the phytotoxins. In addition, our findings revealed that three hub genes commonly induced by both phytotoxins function as the siderophore enterobactin, an ironchelator. Our study provides new insights into the effects of phytotoxins on bacteria for better understanding of the interactions between phytopathogens and other microorganisms. These data will also be applied as a valuable source in subsequent applications against phytotoxins, the major virulence factor.

Keywords: transcriptome;toxoflavin;tropolone;phytopathogenic Burkholderia;Escherichia coli

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Transcriptome analysis to understand the effects of the toxoflavin and tropolone produced by phytopathogenic Burkholderia on Escherichia coli

J. Microbiol. 2019;57(9):781-794. Published online August 27, 2019

DOI: https://doi.org/10.1007/s12275-019-9330-1

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