Diluted conventional media improve the microbial cultivability from aquarium seawater

Jing Sun; Jiazhen Guo; Qian Yang; Jie Huang

doi:10.1007/s12275-019-9175-7

Articles

Page Path: HOME > J. Microbiol > Volume 57(9); 2019 > Article

Journal Article
Diluted conventional media improve the microbial cultivability from aquarium seawater: Jing Sun ¹, Jiazhen Guo ², Qian Yang ¹, Jie Huang ¹; Journal of Microbiology 2019;57(9):759-768.
DOI: https://doi.org/10.1007/s12275-019-9175-7
Published online: August 3, 2019

¹Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P. R. China, ²Qingdao Aquarium, Qingdao 266003, P. R. China

Corresponding author: Jie Huang , Tel: +86-13805421513,

Received: 4 April 2019 • Revised: 15 May 2019 • Accepted: 20 May 2019

prev next

249 Views
0 Download
12 Web of Science
11 Crossref
13 Scopus

Full Article

Download PDF

Abstract

The cultivation of microbial species remains a primary challenge in microbiology and obtaining pure cultures is essential for the study of microbial physiology and function. When isolating microorganisms from aquaculture environments, Vibrio are the most dominate isolates on the media that are commonly used. In order to expand our ability to study microbial species, an easy-operation and low-cost medium that can reduce the interference of Vibrio strains and increase the cultivability of other bacteria is urgently needed. We compared viable cell counts on conventional media (CM; including Marine Agar 2216 and LB media) and diluted media (DM; including 1/10-Marine Agar 2216, 1/10-LB). We also assessed the diversity of cultivable microorganisms under high and low nutrient conditions by a plate-wash strategy coupled with high-throughput sequencing of the V4 hypervariable region of the 16S rRNA gene. The results show that microbial communities from DM, especially 1/10-Marine Agar 2216, are more diverse than those obtained from CM. Vibrio isolates were reduced on DM. PICRUSt analysis revealed that nutrient composition is a significant contributor to the diversity and function of the cultivable microbial communities. Bacteria grown on CM possess more pathogenic characteristics, whereas DM favors the growth of bacteria that have multiple metabolic functions. Collectively, our data provide strong evidence that dilution of CM influences the cultivability of bacteria from aquaculture seawater. It also supports that DM can expand the range of microbial species that can be cultivated. This study also provides insights for media design in microbial cultivation from aquaculture systems.

Keywords: diluted media;microbial cultivability;aquarium seawater

Figure & Data

References

Citations

Citations to this article as recorded by

In vitro domestication of halophyte microbiota for future SynCom application
Nada A. Moner, Tarek R. Elsayed, Wafaa M. Amer, Mervat A. Hamza, Hanan H. Youssef, Randa M. Abdel-Fatah, Mahmoud S. Abdelwahab, Omar M. Shahat, Gehan H. Youssef, Mahmoud El-Tahan, Mohamed Fayez, Florian Fricke, Silke Ruppel, Nabil A. Hegazi
Plant and Soil.2025;[Epub] CrossRef
Heterologous Expression and Functional Analysis of Exiguobacterium Algin Lyase Gene by Pichia pastoris
Hanwen Wu, Kai Hou, Yutong Jiang, Mingjian Luan, Yuxia Sun, Xi He, Xiangzhong Zhao
Fermentation.2025; 11(1): 34. CrossRef
Microbial diversity and ecological roles of halophilic microorganisms in Dingbian (Shaanxi, China) saline-alkali soils and salt lakes
Yue Ding, Juntao Ke, Tao Hong, Aodi Zhang, Xue Wu, Xinran Jiang, Shilong Shao, Ming Gong, Shengda Zhao, Liang Shen, Shaoxing Chen
BMC Microbiology.2025;[Epub] CrossRef
Unexpected methane oxidation acceleration by two species of bacteria from the rainwater promoting Methylomonas sp. in the soil
Yuxuan Sang, Jia Tang, Oumei Wang, Qinqin Hao, Shiling Zheng, Fanghua Liu
Journal of Environmental Chemical Engineering.2024; 12(5): 113895. CrossRef
Glucose addition improves the culture performance of Pacific white shrimp by regulating the assembly of Rhodobacteraceae taxa in gut bacterial community
Pengsheng Dong, Haipeng Guo, Lei Huang, Demin Zhang, Kai Wang
Aquaculture.2023; 567: 739254. CrossRef
Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons
Gabriela Kapinusova, Marco A. Lopez Marin, Ondrej Uhlik
Frontiers in Microbiology.2023;[Epub] CrossRef
The Metabolomics Changes in Luria–Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth
Haifeng Wang, Juan Guo, Xing Chen, Hongxuan He
Metabolites.2023; 13(8): 958. CrossRef
Conjugative Transfer of Acute Hepatopancreatic Necrosis Disease-Causing pVA1-Type Plasmid Is Mediated by a Novel Self-Encoded Type IV Secretion System
Dehao Wang, Liying Wang, Dexi Bi, Jipeng Song, Guohao Wang, Ye Gao, Kathy F. J. Tang, Fanzeng Meng, Jingmei Xie, Fan Zhang, Jie Huang, Jianliang Li, Xuan Dong, Jennifer M. Auchtung, Songzhe Fu
Microbiology Spectrum.2022;[Epub] CrossRef
Culturomics of Bacteria from Radon-Saturated Water of the World’s Oldest Radium Mine
Gabriela Kapinusova, Kunal Jani, Tereza Smrhova, Petr Pajer, Irena Jarosova, Jachym Suman, Michal Strejcek, Ondrej Uhlik, Jing Han
Microbiology Spectrum.2022;[Epub] CrossRef
Diluted Luria-Bertani medium vs. sewage sludge as growth media: comparison of community structure and diversity in the culturable bacteria
Kazuo Yamamoto, Shotaro Toya, Sarah Sabidi, Yuki Hoshiko, Toshinari Maeda
Applied Microbiology and Biotechnology.2021; 105(9): 3787. CrossRef
Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota
Hend Elsawey, Sascha Patz, Rahma A. Nemr, Mohamed S. Sarhan, Mervat A. Hamza, Hanan H. Youssef, Mohamed R. Abdelfadeel, Hassan-Sibroe A. Daanaa, Mahmoud El-Tahan, Mohamed Abbas, Mohamed Fayez, Katja Witzel, Silke Ruppel, Nabil A. Hegazi
Diversity.2020; 12(11): 418. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Diluted conventional media improve the microbial cultivability from aquarium seawater

J. Microbiol. 2019;57(9):759-768. Published online August 3, 2019

DOI: https://doi.org/10.1007/s12275-019-9175-7

Related articles