RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli

Jaejin Lee; Dong-Ho Lee; Che Ok Jeon; Kangseok Lee

doi:10.1007/s12275-019-9354-6

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RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli: Jaejin Lee , Dong-Ho Lee , Che Ok Jeon , Kangseok Lee; Journal of Microbiology 2019;57(10):910-917.
DOI: https://doi.org/10.1007/s12275-019-9354-6
Published online: September 30, 2019

Department of Life Science, Chung-Ang University, Seoul 06974, Republic of KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea

Corresponding author: Che Ok Jeon , Tel: +82-2-820-5241,
Kangseok Lee , Tel: +82-2-820-5241,

Received: 16 July 2019 • Revised: 19 July 2019 • Accepted: 19 July 2019

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Abstract

Studies have shown that many enzymes involved in glycolysis are upregulated in Escherichia coli endoribonuclease G (rng) null mutants. However, the molecular mechanisms underlying the RNase G-associated regulation of glycolysis have not been characterized. Here, we show that RNase G cleaves the 5􍿁􀁇untranslated region of triosephosphate isomerase A (tpiA) mRNA, leading to destabilization of the mRNA in E. coli. Nucleotide substitutions within the RNase G cleavage site in the genome resulted in altered tpiA mRNA stability, indicating that RNase G activity influences tpiA mRNA abundance. In addition, we observed that tpiA expression was enhanced, whereas that of RNase G was decreased, in E. coli cells grown anaerobically. Our findings suggest that RNase G negatively regulates tpiA mRNA abundance in response to oxygen availability in E. coli.

Keywords: glycolysis;mRNA abundance;rng;RNase G;tpiA

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RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli

J. Microbiol. 2019;57(10):910-917. Published online September 30, 2019

DOI: https://doi.org/10.1007/s12275-019-9354-6

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