Comparative portrayal of ocular surface microbe with and without dry eye

ZhenHao Li; Yufang Gong; ShuZe Chen; SiQi Li; Yu Zhang; HuiMin Zhong; ZhouCheng Wang; YiFan Chen; QiXin Deng; YuTing Jiang; LiYing Li; Min Fu; GuoGuo Yi

doi:10.1007/s12275-019-9127-2

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Comparative portrayal of ocular surface microbe with and without dry eye: ZhenHao Li ¹, Yufang Gong ², ShuZe Chen ¹, SiQi Li ¹, Yu Zhang ¹, HuiMin Zhong ¹, ZhouCheng Wang ¹, YiFan Chen ¹, QiXin Deng ¹, YuTing Jiang ¹, LiYing Li ³, Min Fu ¹, GuoGuo Yi ¹; Journal of Microbiology 2019;57(11):1025-1032.
DOI: https://doi.org/10.1007/s12275-019-9127-2
Published online: August 28, 2019

¹Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, P. R. China, ²School of Nursing, Hunan University of Medicine, Huaihua, Hunan, P. R. China, ³Department of Ophthalmology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, P. R. China¹Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, P. R. China, ²School of Nursing, Hunan University of Medicine, Huaihua, Hunan, P. R. China, ³Department of Ophthalmology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, P. R. China

Corresponding author: Min Fu , Tel: +86-188191316103,
GuoGuo Yi , Tel: +86-188191316103,

Received: 9 March 2019 • Revised: 14 July 2019 • Accepted: 15 July 2019

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Abstract

To compare the ocular surface (OS) microbial communities and diversity between dry eye (DE) and non-DE (NDE). Furthermore, we compared meibomian gland dysfunction (MGD) and non-MGD (NMGD) among DE subjects. The V3-V4 region of 16S rRNA gene high-throughput sequencing was performed in the conjunctival swab samples to investigate the composition of the OS bacterial community in DE (n=35) and NDE (n=54) and compared the composition of MGD (n=25) and NMGD (n=10) among DE subjects. Deep sequencing of OS 16S rDNA from DE (n=35) and NDE (n=54) demonstrated great a difference in alpha and beta diversity between the OS bacterial flora (P < 0.05). The similar OS microbial structures were shown at the phylum and genus levels by bioinformatics analysis between them, and in LEfSe (linear discriminant analysis effect size) analysis, Bacteroidia and Bacteroidetes were enriched in DE, while Pseudomonas was plentiful in NDE (linear discriminant analysis [LDA] > 4.0). Among the DE group, there was no significant difference in α and β diversity between MGD and NMGD (P > 0.05). Surprisingly, Bacilli was the dominant microbe in MGD, and Bacteroidetes was the superior bacteria in NMGD among DE subjects (LDA > 4.0). Different diversity of OS bacteria composition between DE and NDE and the altered diversity of OS bacteria may play an important role in DE. Moreover, the lower dominance of OS bacteria in DE may be associated with the occurrence and development of DE. Although there was no significant difference in alpha and beta analysis, the OS dominant microbe between MGD and NMGD among DE was different.

Keywords: dry eye;meibomian gland dysfunction;ocular surface bacteria;16S rRNA gene sequencing

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Comparative portrayal of ocular surface microbe with and without dry eye

J. Microbiol. 2019;57(11):1025-1032. Published online August 28, 2019

DOI: https://doi.org/10.1007/s12275-019-9127-2

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