Exploring the antibiotic resistome in activated sludge and anaerobic digestion sludge in an urban wastewater treatment plant via metagenomic analysis

Keunje Yoo; Hyunji Yoo; Jangho Lee; Eun Joo Choi; Joonhong Park

doi:10.1007/s12275-020-9309-y

Articles

Page Path: HOME > J. Microbiol > Volume 58(2); 2020 > Article

Journal Article
Exploring the antibiotic resistome in activated sludge and anaerobic digestion sludge in an urban wastewater treatment plant via metagenomic analysis: Keunje Yoo ¹, Hyunji Yoo ², Jangho Lee ², Eun Joo Choi ³, Joonhong Park ²; Journal of Microbiology 2020;58(2):123-130.
DOI: https://doi.org/10.1007/s12275-020-9309-y
Published online: December 23, 2019

¹Department of Environmental Engineering, Korea Maritime and Ocean University, Busan 49112, Republic of Korea, ²Department of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea, ³Department of Systems Biology, Yonsei University, Seoul 03722, Republic of Korea¹Department of Environmental Engineering, Korea Maritime and Ocean University, Busan 49112, Republic of Korea, ²Department of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea, ³Department of Systems Biology, Yonsei University, Seoul 03722, Republic of Korea

Corresponding author: Joonhong Park , Tel: +82-2-2123-5798,

Received: 20 June 2019 • Revised: 8 October 2019 • Accepted: 3 November 2019

prev next

46 Views
0 Download
52 Web of Science
49 Crossref
49 Scopus

Full Article

Abstract

Antibiotic resistance genes (ARGs) are emerging contaminants that pose a potential threat to human health worldwide. Urban wastewater treatment plants (WWTPs) are a main source of both antibiotic-resistant bacteria and ARGs released into the environment. Nevertheless, the propagation of ARGs and their underlying mechanisms and the dynamics of mobile genetic elements (MGEs) in WWTPs have rarely been investigated in South Korea. In this study, shotgun metagenomic analysis was used to identify comprehensive ARGs and their mechanisms, bacterial communities, and MGEs from 4 configurations with 2 activated sludge (AS) and 2 anaerobic digestion sludge (ADS) samples. A total of 181 ARG subtypes belonging to 22 ARG types were broadly detected, and the ARG abundances in the AS samples were 1.3–2.0 orders of magnitude higher than in the ADS samples. Multidrug and bacitracin resistance genes were the predominant ARG types in AS samples, followed by ARGs against sulfonamide, tetracycline, and β-lactam. However, the composition of ARG types in ADS samples was significantly changed. The abundance of multidrug and β-lactam resistance genes was drastically reduced in the ADS samples. The resistance genes of MLS were the predominant, followed by ARGs against sulfonamide and tetracycline in the ADS samples. In addition, plasmids were the dominant MGEs in the AS samples, while integrons (intI1) were the dominant MGEs in the ADS samples. These results provide valuable information regarding the prevalence of ARG types and MGEs and the difference patterns between the AS and ADS systems.

Keywords: antibiotic resistance genes (ARGs);Mobile genetic elements;urban wastewater treatment plant;metagenomics

Figure & Data

References

Citations

Citations to this article as recorded by

Spatiotemporal distribution of the planktonic microbiome and antibiotic resistance genes in a typical urban river contaminated by macrolide antibiotics
Chuanmao Yang, Shiwei Yan, Baihuan Zhang, Xiunan Yao, Jiezhang Mo, Fozia Rehman, Jiahua Guo
Environmental Research.2024; 262: 119808. CrossRef
Resistomes in freshwater bioaerosols and their impact on drinking and recreational water safety: A perspective
Salametu Saibu, Ishara Uhanie Perera, Satoru Suzuki, Xavier Rodó, So Fujiyoshi, Fumito Maruyama
Environment International.2024; 183: 108377. CrossRef
An evaluation of conventional and nature-based technologies for controlling antibiotic-resistant bacteria and antibiotic-resistant genes in wastewater treatment plants
Moushumi Hazra, Joy E.M. Watts, John B. Williams, Himanshu Joshi
Science of The Total Environment.2024; 917: 170433. CrossRef
Sewage sludge: A review of its risks and circular raw material potential
Halyna Kominko, Katarzyna Gorazda, Zbigniew Wzorek
Journal of Water Process Engineering.2024; 63: 105522. CrossRef
Genome-centric analyses of 165 metagenomes show that mobile genetic elements are crucial for the transmission of antimicrobial resistance genes to pathogens in activated sludge and wastewater
Nafi’u Abdulkadir, Joao Pedro Saraiva, Junya Zhang, Stefan Stolte, Osnat Gillor, Hauke Harms, Ulisses Rocha, Adriana E. Rosato
Microbiology Spectrum.2024;[Epub] CrossRef
A review of wastewater-based epidemiology for antimicrobial resistance surveillance
Leah M. Clarke, Jake W. O’Brien, Aimee K. Murray, William H. Gaze, Kevin V. Thomas
Journal of Environmental Exposure Assessment.2024;[Epub] CrossRef
Antibiotic Resistance Genes – An Emerging Genetic Pollutant of LFL
Jennet Debora J., Vijayalakshmi V., Rajaguru P., Rajeswari S., Brindha R.
Water, Air, & Soil Pollution.2024;[Epub] CrossRef
Unveiling the hidden hazards of smog: health implications and antibiotic resistance in perspective
Muhammad Imran Khan, Arshia Amin, Muhammad Tariq Khan, Hafsa Jabeen, Shafqat Rasul Chaudhry
Aerobiologia.2024; 40(3): 353. CrossRef
Marine plastisphere selectively enriches microbial assemblages and antibiotic resistance genes during long-term cultivation periods
Hyunsu Kim, Keunje Yoo
Environmental Pollution.2024; 344: 123450. CrossRef
Microbial symbiotic electrobioconversion of carbon dioxide to biopolymer (poly (3-hydroxybutyrate)) via single-step microbial electrosynthesis cell
Giang T.H. Le, Hend Omar Mohamed, Hyunsu Kim, Keunje Yoo, Tasnim Eisa, Dipak A. Jadhav, Ha T.T. Nguyen, Hyerim Eam, Jaewook Myung, Pedro Castaño, Kyu-Jung Chae
Chemical Engineering Journal.2024; 500: 156635. CrossRef
Performance of sewage sludge treatment for the removal of antibiotic resistance genes: Status and prospects
Shiyu Yin, Le Gao, Xiumin Fan, Shuhong Gao, Xu Zhou, Wenbiao Jin, Zhongqi He, Qilin Wang
Science of The Total Environment.2024; 907: 167862. CrossRef
Impacts of microplastic type on the fate of antibiotic resistance genes and horizontal gene transfer mechanism during anaerobic digestion
Ming Xu, Peng Gao, Yuan Gao, Shi-jin Xiong, Hao-qiang Chen, Xiao-xiao Shen
Journal of Environmental Management.2024; 360: 121090. CrossRef
Quaternary ammonia compounds in disinfectant products: evaluating the potential for promoting antibiotic resistance and disrupting wastewater treatment plant performance
Zihao Lu, Anna K. Mahony, William A. Arnold, Christopher W. Marshall, Patrick J. McNamara
Environmental Science: Advances.2024; 3(2): 208. CrossRef
Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples
Xiaole Yin, Xi Chen, Xiao-Tao Jiang, Ying Yang, Bing Li, Marcus Ho-Hin Shum, Tommy T. Y. Lam, Gabriel M. Leung, Joan Rose, Concepcion Sanchez-Cid, Timothy M. Vogel, Fiona Walsh, Thomas U. Berendonk, Janet Midega, Chibuzor Uchea, Dominic Frigon, Gerard D.
Environmental Science & Technology.2023; 57(26): 9713. CrossRef
Catch me if you can: capturing microbial community transformation by extracellular DNA using Hi-C sequencing
David Calderón-Franco, Mark C. M. van Loosdrecht, Thomas Abeel, David G. Weissbrodt
Antonie van Leeuwenhoek.2023; 116(7): 667. CrossRef
Effects of micron-scale zero valent iron on behaviors of antibiotic resistance genes and pathogens in thermophilic anaerobic digestion of waste activated sludge
Wenqian Li, Lina Pang, Efthalia Chatzisymeon, Ping Yang
Bioresource Technology.2023; 376: 128895. CrossRef
Exploring antibiotic resistance genes, mobile gene elements, and virulence gene factors in an urban freshwater samples using metagenomic analysis
Sohail M. Noman, Muhammad Shafiq, Shabana Bibi, Bharti Mittal, Yumeng Yuan, Mi Zeng, Xin Li, Oluwaseyi Abraham Olawale, Xiaoyang Jiao, Muhammad Irshad
Environmental Science and Pollution Research.2023; 30(2): 2977. CrossRef
Mining-related multi-resistance genes in sulfate-reducing bacteria treatment of typical karst nonferrous metal(loid) mine tailings in China
Jian-li Liu, Jun Yao, De-liang Zhou, Bang Liu, Houquan Liu, Miaomiao Li, Chenchen Zhao, Geoffrey Sunahara, Robert Duran
Environmental Science and Pollution Research.2023; 30(47): 104753. CrossRef
Variations in antibiotic resistance genes and microbial community in sludges passing through biological nutrient removal and anaerobic digestion processes in municipal wastewater treatment plants
Shahbaz Raza, Kyeong Hwan Kang, Juhee Shin, Seung Gu Shin, Jihyun Chun, Hyun Uk Cho, Jingyeong Shin, Young Mo Kim
Chemosphere.2023; 313: 137362. CrossRef
Drinking water sources as hotspots of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs): Occurrence, spread, and mitigation strategies
Shengnan Li, Brim Stevy Ondon, Shih-Hsin Ho, Qixing Zhou, Fengxiang Li
Journal of Water Process Engineering.2023; 53: 103907. CrossRef
Co-Existence and Proliferation of Heavy Metal and Antibiotic Resistance in Urban Sewage Treatment Plants
Sonia Gupta, T. R. Sreekrishnan, Shaikh Ziauddin Ahammad
SSRN Electronic Journal .2022;[Epub] CrossRef
Mining-Related Multi-Resistance Genes in Typical Nonferrous Metal(Loid) Tailing Impoundments Under Bio-Restoration
Jianli Liu, Jun Yao, Deliang Zhou, Hao Li, Bang Liu, Houquan Liu, Miaomiao Li, Chenchen Zhao, Geoffrey Sunahara, Robert Duran
SSRN Electronic Journal .2022;[Epub] CrossRef
A review of the emergence of antibiotic resistance in bioaerosols and its monitoring methods
Gihan Lee, Keunje Yoo
Reviews in Environmental Science and Bio/Technology.2022; 21(3): 799. CrossRef
Lime addition significantly attenuates tetracycline resistance genes and class 1 integrons in dewatered sludge by affecting bacterial profiles
Maoxia Chen, Jiali Chang, Tao Jiang, Shiqian Wei, Jiawei Zou, Juan Yang, Xuguang Ma
Journal of Environmental Chemical Engineering.2022; 10(3): 107429. CrossRef
Fate of antibiotic resistant genes in wastewater environments and treatment strategies - A review
Ranjith Kumar Manoharan, Fahmida Ishaque, Young-Ho Ahn
Chemosphere.2022; 298: 134671. CrossRef
mobileOG-db: a Manually Curated Database of Protein Families Mediating the Life Cycle of Bacterial Mobile Genetic Elements
Connor L. Brown, James Mullet, Fadi Hindi, James E. Stoll, Suraj Gupta, Minyoung Choi, Ishi Keenum, Peter Vikesland, Amy Pruden, Liqing Zhang, Hideaki Nojiri
Applied and Environmental Microbiology.2022;[Epub] CrossRef
The prevalence and removal of antibiotic resistance genes in full-scale wastewater treatment plants: Bacterial host, influencing factors and correlation with nitrogen metabolic pathway
Qian Ping, Zhipeng Zhang, Liping Ma, Tingting Yan, Lin Wang, Yongmei Li
Science of The Total Environment.2022; 827: 154154. CrossRef
Effects of heavy metals on the development and proliferation of antibiotic resistance in urban sewage treatment plants
Sonia Gupta, T.R. Sreekrishnan, Shaikh Ziauddin Ahammad
Environmental Pollution.2022; 308: 119649. CrossRef
Mining-Related Multi-Resistance Genes in Typical Nonferrous Metal(Loid) Tailing Impoundments Under Bio-Restoration
Jianli Liu, Jun Yao, Deliang Zhou, Hao Li, Bang Liu, Houquan Liu, Miaomiao Li, Chenchen Zhao, Geoffrey Sunahara, Robert Duran
SSRN Electronic Journal .2022;[Epub] CrossRef
Genetic and genomic characterization of multidrug resistant Bacillus subtilis M3 isolated from an activated sludge reactor treating wastewater
Hafeez Ul Haq, Wei Huang, Ye Li, Ting Zhang, Shaocheng Ma, Yajing Zhang, Yongkang Song, Daiyan Lin, Baoyu Tian
Biologia.2022; 77(4): 1151. CrossRef
Wastewater treatment plants as reservoirs and sources for antibiotic resistance genes: A review on occurrence, transmission and removal
Yu-Xi Gao, Xing Li, Xiao-Yan Fan, Jun-Ru Zhao, Zhong-Xing Zhang
Journal of Water Process Engineering.2022; 46: 102539. CrossRef
Metagenomics analysis of probable transmission of determinants of antibiotic resistance from wastewater to the environment – A case study
Wiktor Zieliński, Jakub Hubeny, Martyna Buta-Hubeny, Damian Rolbiecki, Monika Harnisz, Łukasz Paukszto, Ewa Korzeniewska
Science of The Total Environment.2022; 827: 154354. CrossRef
Impact of Anthropogenic Activities on the Dissemination of ARGs in the Environment—A Review
Małgorzata Czatzkowska, Izabela Wolak, Monika Harnisz, Ewa Korzeniewska
International Journal of Environmental Research and Public Health.2022; 19(19): 12853. CrossRef
Higher abundance of core antimicrobial resistant genes in effluent from wastewater treatment plants
Shahbaz Raza, Hanseob Shin, Hor-Gil Hur, Tatsuya Unno
Water Research.2022; 208: 117882. CrossRef
Metagenomic Analysis Reveals the Fate of Antibiotic Resistance Genes in a Full-Scale Wastewater Treatment Plant in Egypt
Osama S. Ali, Walaa G. Hozayen, Abdulwahab S. Almutairi, Sherif A. Edris, Aala A. Abulfaraj, Amged A. Ouf, Hamada M. Mahmoud
Sustainability.2021; 13(20): 11131. CrossRef
Shotgun metagenomic analysis reveals the prevalence of antibiotic resistance genes and mobile genetic elements in full scale hospital wastewater treatment plants
Ranjith Kumar Manoharan, Sathiyaraj Srinivasan, Gnanendra Shanmugam, Young-Ho Ahn
Journal of Environmental Management.2021; 296: 113270. CrossRef
Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
Ana R. Cardoso, Liliana P.T. Carneiro, Gustavo Cabral-Miranda, Martin F. Bachmann, M. Goreti F. Sales
Chemical Engineering Journal.2021; 409: 128135. CrossRef
Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective
Misung Kim, Jaeeun Park, Mingyeong Kang, Jihye Yang, Woojun Park
Journal of Microbiology.2021; 59(6): 535. CrossRef
Monitoring antibiotic resistance genes in wastewater treatment: Current strategies and future challenges
Anh Q. Nguyen, Hang P. Vu, Luong N. Nguyen, Qilin Wang, Steven P. Djordjevic, Erica Donner, Huabing Yin, Long D. Nghiem
Science of The Total Environment.2021; 783: 146964. CrossRef
Agricultural Soils Amended With Thermally-Dried Anaerobically-Digested Sewage Sludge Showed Increased Risk of Antibiotic Resistance Dissemination
Leire Jauregi, Lur Epelde, Itziar Alkorta, Carlos Garbisu
Frontiers in Microbiology.2021;[Epub] CrossRef
Antibiotic resistome from the One-Health perspective: understanding and controlling antimicrobial resistance transmission
Dae-Wi Kim, Chang-Jun Cha
Experimental & Molecular Medicine.2021; 53(3): 301. CrossRef
Occurrence and Distribution of Antibiotic Resistance Genes in Municipal Wastewater Treatment Plants with D-Type Filters
Haoze Wang, Bing Li, Jiaheng Zhao, Yongjing Tian, Yong Qiu
Water.2021; 13(23): 3398. CrossRef
Mining-Related Multi-Resistance Genes in Typical Karst Nonferrous Metal(Loid) Tailing Impoundments Under Bio-Restoration
Jianli Liu, Jun Yao, Deliang Zhou, Hao Li, Bang Liu, Weihua Cui, Xinyuan Li, Hang Meng, Robert Duran
SSRN Electronic Journal .2021;[Epub] CrossRef
Role of anaerobic sludge digestion in handling antibiotic resistant bacteria and antibiotic resistance genes – A review
Achmad Syafiuddin, Raj Boopathy
Bioresource Technology.2021; 330: 124970. CrossRef
Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis
Keunje Yoo, Gihan Lee
Antibiotics.2021; 10(2): 188. CrossRef
Remediating oxytetracycline-contaminated aquaculture water using nano calcium peroxide (nCaO2) produced from flue gas desulfurization (FGD) gypsum
Piyarat Vijuksungsith, Tunlawit Satapanajaru, Chanat Chokejaroenrat, Chalor Jarusutthirak, Chainarong Sakulthaew, Ann Kambhu, Rattana Boonprasert
Environmental Technology & Innovation.2021; 24: 101861. CrossRef
Metagenomic exploration of antibiotic resistome in treated wastewater effluents and their receiving water
Shahbaz Raza, Hyejun Jo, Jungman Kim, Hanseob Shin, Hor-Gil Hur, Tatsuya Unno
Science of The Total Environment.2021; 765: 142755. CrossRef
Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context
Francisco Cerqueira, Anastasis Christou, Despo Fatta-Kassinos, Maria Vila-Costa, Josep Maria Bayona, Benjamin Piña
Journal of Hazardous Materials.2020; 400: 123208. CrossRef
Metagenomic Profiles of Antibiotic Resistance Genes in Activated Sludge, Dewatered Sludge and Bioaerosols
Il Han, Keunje Yoo
Water.2020; 12(6): 1516. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Exploring the antibiotic resistome in activated sludge and anaerobic digestion sludge in an urban wastewater treatment plant via metagenomic analysis

J. Microbiol. 2020;58(2):123-130. Published online December 23, 2019

DOI: https://doi.org/10.1007/s12275-020-9309-y

Related articles