Optimization of bacterial sporulation using economic nutrient for self-healing concrete

Youngung Ryu; Ki-Eun Lee; In-Tae Cha; Woojun Park

doi:10.1007/s12275-020-9580-y

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Optimization of bacterial sporulation using economic nutrient for self-healing concrete: Youngung Ryu ¹, Ki-Eun Lee ², In-Tae Cha ², Woojun Park ¹; Journal of Microbiology 2020;58(4):288-296.
DOI: https://doi.org/10.1007/s12275-020-9580-y
Published online: February 27, 2020

¹Laboratory of Molecular Environmental Microbiology, Department of Environmental Sciences and Ecological Engineering, Korea University,Seoul 02841, Republic of Korea , ²National Institute of Biological Resources, Incheon, 22689, Republic of Korea¹Laboratory of Molecular Environmental Microbiology, Department of Environmental Sciences and Ecological Engineering, Korea University,Seoul 02841, Republic of Korea , ²National Institute of Biological Resources, Incheon, 22689, Republic of Korea

Corresponding author: Woojun Park , Tel: +82-2-3290-3067,

Received: 4 December 2019 • Revised: 14 January 2020 • Accepted: 29 January 2020

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Abstract

The use of heat- and alkali-resistant bacteria is essential for the biological repair of damaged concrete. Lysinibacillus boronitolerans YS11 was isolated from the rhizosphere of Miscanthus sacchariflorus. The increased pH in the urea-minus condition during the growth of the YS11 strain promoted calcium carbonate (CaCO3) formation. To identify the optimum medium that promoted the growth of the YS11 strain, a Plackett- Burman design was conducted for the screening process. Consequently, malt powder, rice bran, (NH4)2SO4, and corn syrup were chosen to enhance YS11 growth. The optimization of these four useful factors was carried out using a central composite design. To obtain higher survivability in mortar, the sporulation process is essential, and additional factors such as Mn2+, Fe2+, and Ca2+ were found to contribute to sporulation. A mixture of L. boronitolerans YS11 spore powder, cement, paste, sand, yeast extract, calcium lactate, and water showed a healing effect on a 0.3 mm mortar crack in 7 days. Furthermore, calcium carbonate precipitation was observed over the crack surface. Thus, we confirmed that mortar treated with YS11 spore powder was effective in healing micro-cracks in concrete.

Keywords: Lysinibacillus boronitolerans YS1;MICP;calcium carbonate;concrete healing;economic media

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Optimization of bacterial sporulation using economic nutrient for self-healing concrete

J. Microbiol. 2020;58(4):288-296. Published online February 27, 2020

DOI: https://doi.org/10.1007/s12275-020-9580-y

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