[Protocol] Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

Yoontak Han; Eun-Jin Lee

doi:10.1007/s12275-020-9297-y

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[Protocol] Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry: Yoontak Han , Eun-Jin Lee; Journal of Microbiology 2020;58(4):245-251.
DOI: https://doi.org/10.1007/s12275-020-9297-y
Published online: November 23, 2019

Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of KoreaDepartment of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea

Corresponding author: Eun-Jin Lee , Tel: +82-2-3290-3411,

Received: 18 June 2019 • Revised: 11 October 2019 • Accepted: 20 October 2019

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Abstract

The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.

Keywords: Salmonella Typhimurium;Type II flagella

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[Protocol] Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

J. Microbiol. 2020;58(4):245-251. Published online November 23, 2019

DOI: https://doi.org/10.1007/s12275-020-9297-y

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