Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells

Jagoda Płaczkiewicz; Paulina Chmiel; Ewelina Malinowska; Pawel B&#; Agnieszka Kwiatek

doi:10.1007/s12275-020-9505-9

Articles

Page Path: HOME > J. Microbiol > Volume 58(5); 2020 > Article

Journal Article
Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells: Jagoda Płaczkiewicz ¹, Paulina Chmiel ¹, Ewelina Malinowska ¹, Pawel B&# ^261;cal2, Agnieszka Kwiatek ¹; Journal of Microbiology 2020;58(5):405-414.
DOI: https://doi.org/10.1007/s12275-020-9505-9
Published online: April 11, 2020

¹Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland, ²Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Ks. Trojdena 4, 02-109 Warsaw, Poland¹Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland, ²Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Ks. Trojdena 4, 02-109 Warsaw, Poland

Corresponding author: Agnieszka Kwiatek , Tel: +48-22-55-41-421,

Received: 28 October 2019 • Revised: 30 January 2020 • Accepted: 30 January 2020

prev next

75 Views
0 Download
10 Web of Science
8 Crossref
11 Scopus

Full Article

Abstract

Neisseria gonorrhoeae, an obligatory human pathogen causes the sexually transmitted disease gonorrhea, which remains a global health problem. N. gonorrhoeae primarily infects the mucosa of the genitourinary tract, which in women, is colonized by natural microbiota, dominated by Lactobacillus spp., that protect human cells against pathogens. In this study, we demonstrated that precolonization of human epithelial cells with Lactobacillus crispatus, one of the most prevalent bacteria in the female urogenital tract, or preincubation with the L. crispatus enolase or glutamine synthetase impairs the adhesion and invasiveness of N. gonorrhoeae toward epithelial cells, two crucial steps in gonococcal pathogenesis. Furthermore, decreased expression of genes encoding the proinflammatory cytokines, TNFα and CCL20, which are secreted as a consequence of N. gonorrhoeae infection, was observed in N. gonorrhoeae-infected epithelial cells that had been precolonized with L. crispatus or preincubated with enolase and glutamine synthetase. Thus, our results indicate that the protection of human cells against N. gonorrhoeae infection is a complex process and that L. crispatus and its proteins enolase and glutamine synthetase can have a potential role in protecting epithelial cells against gonococcal infection. Therefore, these results are important since disturbances of the microbiota or of its proteins can result in dysbiosis, which is associated with increased susceptibility of epithelium to pathogens.

Keywords: Neisseria gonorrhoeae;enolase;glutamine synthetase;microbiota;Lactobacillus crispatus

Figure & Data

References

Citations

Citations to this article as recorded by

Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract
Li Ying Jessie Lau, Siew Young Quek
Food Bioengineering.2024; 3(1): 41. CrossRef
Use of probiotic lactobacilli in the treatment of vaginal infections: In vitro and in vivo investigations
Peng Liu, Yune Lu, Rongguo Li, Xiaodi Chen
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Enhanced IgA coating of bacteria in women with Lactobacillus crispatus-dominated vaginal microbiota
Annelot C. Breedveld, Heleen J. Schuster, Robin van Houdt, Rebecca C. Painter, Reina E. Mebius, Charlotte van der Veer, Sylvia M. Bruisten, Paul H. M. Savelkoul, Marjolein van Egmond
Microbiome.2022;[Epub] CrossRef
Molecular Regulatory Mechanisms Drive Emergent Pathogenetic Properties of Neisseria gonorrhoeae
Ashwini Sunkavalli, Ryan McClure, Caroline Genco
Microorganisms.2022; 10(5): 922. CrossRef
Both Neisseria gonorrhoeae and Neisseria sicca Induce Cytokine Secretion by Infected Human Cells, but Only Neisseria gonorrhoeae Upregulates the Expression of Long Non-Coding RNAs
Jagoda Płaczkiewicz, Monika Adamczyk-Popławska, Ewa Kozłowska, Agnieszka Kwiatek
Pathogens.2022; 11(4): 394. CrossRef
Role of the human vaginal microbiota in the regulation of inflammation and sexually transmitted infection acquisition: Contribution of the non-human primate model to a better understanding?
Cindy Adapen, Louis Réot, Elisabeth Menu
Frontiers in Reproductive Health.2022;[Epub] CrossRef
Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
Hongliang Chen, Li Wang, Lanhua Zhao, Lipei Luo, Shuling Min, Yating Wen, Wenbo Lei, Mingyi Shu, Zhongyu Li
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components
Lidia Muscariello, Barbara De Siena, Rosangela Marasco
Current Microbiology.2020; 77(12): 3831. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells

J. Microbiol. 2020;58(5):405-414. Published online April 11, 2020

DOI: https://doi.org/10.1007/s12275-020-9505-9

Related articles