Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory

Lingmin Jiang; Dexin Wang; Jung-Sook Lee; Dae-Hyuk Kim; Jae Cheol Jeong; Cha Young Kim; Suk Weon Kim; Jiyoung Lee

doi:10.1007/s12275-020-9294-1

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Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory: Lingmin Jiang ^1,2, Dexin Wang ^2,3, Jung-Sook Lee ¹, Dae-Hyuk Kim ², Jae Cheol Jeong ¹, Cha Young Kim ¹, Suk Weon Kim ¹, Jiyoung Lee ¹; Journal of Microbiology 2020;58(5):357-366.
DOI: https://doi.org/10.1007/s12275-020-9294-1
Published online: March 27, 2020

¹Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea, ²Department of Bioactive Materials, Jeonbuk National University, Jeonju 54896, Republic of Korea, ³Radiation Utilization and Facilities Management Division, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea¹Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea, ²Department of Bioactive Materials, Jeonbuk National University, Jeonju 54896, Republic of Korea, ³Radiation Utilization and Facilities Management Division, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea

Corresponding author: Suk Weon Kim , Tel: +82-63-570-5651,
Jiyoung Lee , Tel: +82-63-570-5651,

Received: 17 June 2019 • Revised: 30 October 2019 • Accepted: 23 January 2020

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Abstract

Strain KSNA2T, a Gram-negative, moderately halophilic, facultatively anaerobic, motile, rod-shaped bacterium, was isolated from the surface-sterilized stem tissue of a beach morning glory (Calystegia soldanella) plant in Chuja Island, Jejudo, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain KSNA2T formed a distinct lineage within the family Enterobacteriaceae, with the highest 16S rRNA gene sequence similarity to Izhakiella australiensis KCTC 72143T (96.2%) and Izhakiella capsodis KCTC 72142T (96.0%), exhibited 95.5– 95.9% similarity to other genera in the family Enterobacteriaceae and Erwiniaceae. Conserved signature indels analysis elucidated that strain KSNA2T was delimited into family Enterobacteriaceae. KSNA2T genome comprises a circular chromosome of 5,182,800 bp with 56.1% G + C content. Digital DNA-DNA relatedness levels between strain KSNA2T and 18 closely related species were 19.3 to 21.1%. Average nucleotide identity values were between 72.0 and 76.7%. Growth of strain KSNA2T was observed at 4 to 45°C (optimum, 25°C) and pH 5.0 to 12.0 (optimum, pH 7.0) in the presence of 0 to 11% (w/v) NaCl (optimum, 0–7%). The major cellular fatty acids (> 10%) were C16:0 followed by summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C17:0 cyclo, and C14:0. The major isoprenoid quinone was ubiquinone-8 (Q-8). With combined phylogenetic, genomic, phenotypic, and chemotaxonomic features, strain KSNA2T represents a novel species of a new genus in the family Enterobacteriaceae, for which the name Jejubacter calystegiae gen. nov., sp. nov. is proposed. The type strain is KSNA2T (= KCTC 72234T = CCTCC AB 2019098T).

Keywords: RNase regulator;RNase stability;post-translational regulation of RNase;proteolysis;trans-acting regulator

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Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory

J. Microbiol. 2020;58(5):357-366. Published online March 27, 2020

DOI: https://doi.org/10.1007/s12275-020-9294-1

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