Omp16, a conserved peptidoglycan-associated lipoprotein, is involved in Brucella virulence in vitro

Feijie Zhi; Dong Zhou; Junmei Li; Lulu Tian; Guangdong Zhang; Yaping Jin; Aihua Wang

doi:10.1007/s12275-020-0144-y

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Omp16, a conserved peptidoglycan-associated lipoprotein, is involved in Brucella virulence in vitro: Feijie Zhi ¹, Dong Zhou ^1,2, Junmei Li ¹, Lulu Tian ¹, Guangdong Zhang ¹, Yaping Jin ^1,2, Aihua Wang ^1,2; Journal of Microbiology 2020;58(9):793-804.
DOI: https://doi.org/10.1007/s12275-020-0144-y
Published online: September 1, 2020

¹College of Veterinary Medicine, Northwest A&F University, Yangling, P. R. China, ²Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, P. R. China

Corresponding author: Yaping Jin ,
Aihua Wang ,

Received: 17 March 2020 • Revised: 2 June 2020 • Accepted: 9 July 2020

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Abstract

Brucella, the bacterial agent of common zoonotic brucellosis, primarily infects specific animal species. The Brucella outer membrane proteins (Omps) are particularly attractive for developing vaccine and improving diagnostic tests and are associated with the virulence of smooth Brucella strains. Omp16 is a homologue to peptidoglycan-associated lipoproteins (Pals), and an omp16 mutant has not been generated in any Brucella strain until now. Very little is known about the functions and pathogenic mechanisms of Omp16 in Brucella. Here, we confirmed that Omp16 has a conserved Pal domain and is highly conserved in Brucella. We attempted to delete omp16 in Brucella suis vaccine strain 2 (B. suis S2) without success, which shows that Omp16 is vital for Brucella survival. We acquired a B. suis S2 Omp16 mutant via conditional complementation. Omp16 deficiency impaired Brucella outer membrane integrity and activity in vitro. Moreover, inactivation of Omp16 decreased bacterial intracellular survival in macrophage RAW 264.7 cells. B. suis S2 and its derivatives induced marked expression of IL-1β, IL-6, and TNF-α mRNA in Raw 264.7 cells. Whereas inactivation of Omp16 in Brucella enhanced IL-1β and IL-6 expression in Raw 264.7 cells. Altogether, these findings show that the Brucella Omp16 mutant was obtained via conditional complementation and confirmed that Omp16 can maintain outer membrane integrity and be involved in bacterial virulence in Brucella in vitro and in vivo. These results will be important in uncovering the pathogenic mechanisms of Brucella.

Keywords: B. suis S2, Omp16, conditional expression, tetracycline, attenuation

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Omp16, a conserved peptidoglycan-associated lipoprotein, is involved in Brucella virulence in vitro

J. Microbiol. 2020;58(9):793-804. Published online September 1, 2020

DOI: https://doi.org/10.1007/s12275-020-0144-y

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