Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope

Xiaoxia Wang; Jing Yang; Yuyuan Huang; Xiaomin Wu; Licheng Wang; Limei Han; Sha Li; Huan Li; Xiaoying Fu; Hai Chen; Xiong Zhu

doi:10.1007/s12275-020-0060-1

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Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope: Xiaoxia Wang ^1,2, Jing Yang ^2,3,4, Yuyuan Huang ⁵, Xiaomin Wu ⁶, Licheng Wang ¹, Limei Han ¹, Sha Li ¹, Huan Li ¹, Xiaoying Fu ¹, Hai Chen ¹, Xiong Zhu ¹; Journal of Microbiology 2020;58(9):734-740
DOI: https://doi.org/10.1007/s12275-020-0060-1
Published online: July 24, 2020

¹Central & Clinical Laboratory of Sanya People’s Hospital, Sanya, Hainan 572000, P. R. China, ²State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, P. R. China, ³Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China, ⁴Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, P. R. China, ⁵Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, P. R. China, ⁶Shaanxi Institute of Zoology, Xi􍿁an 710032, P. R. China¹Central & Clinical Laboratory of Sanya People’s Hospital, Sanya, Hainan 572000, P. R. China, ²State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, P. R. China, ³Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China, ⁴Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, P. R. China, ⁵Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, P. R. China, ⁶Shaanxi Institute of Zoology, Xi􍿁an 710032, P. R. China

Corresponding author: Xiong Zhu ,

Received: 4 February 2020 • Revised: 11 June 2020 • Accepted: 16 June 2020

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Abstract

Two aerobic, Gram-stain-positive, non-motile, non-sporulating coccoid strains, designated ZLJ0423T and ZLJ0321, were isolated from the faeces of Tibetan antelope (Pantholops hodgsonii). Their optimal temperature, NaCl concentration and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ZLJ0423T and ZLJ0321 were very similar to each other (99.8%) and had a sequence similarity of 97.0% with Georgenia satyanarayanai NBRC 107612T and Georgenia subflava CGMCC 1.12782T. Phylogenomic analysis based on 688 core genes indicated that these strains formed a clade with G. satyanarayanai NBRC 107612T and Georgenia wutianyii Z294T. The predominant cellular fatty acids were anteiso-C15:0, anteiso-C15:1 A and C16:0. The major menaquinone was MK-8(H4). The cell-wall amino acids consisted of alanine, lysine, glycine and aspartic acid, with lysine as the diagnostic diamino acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified lipids formed the polar lipid profile. The DNA G + C content of both isolates was 73.9 mol%. The digital DNA–DNA hybridization value between strains ZLJ0423T and ZLJ0321 was 91.2%, but their values with closely related species and other available type strains of the genus Georgenia were lower than the 70% threshold. On the basis of polyphasic taxonomic data, strains ZLJ0423T and ZLJ0321 represent a novel species within the genus Georgenia, for which the name Georgenia faecalis sp. nov. is proposed. The type strain is ZLJ0423T (= CGMCC 1.13681T = JCM 33470T).

Keywords: biocatalyst;bioconversion;phosphate sugar isomerase;rare sugar;substrate specificity

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Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope

J. Microbiol. 2020;58(9):734-740. Published online July 24, 2020

DOI: https://doi.org/10.1007/s12275-020-0060-1

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