Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae

Xi Zhang; Yingli Liu; Jing Wang; Yunying Zhao; Yu Deng

doi:10.1007/s12275-020-0261-7

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Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae: Xi Zhang ^1,3, Yingli Liu ², Jing Wang ², Yunying Zhao ^1,4, Yu Deng ^1,3,4; Journal of Microbiology 2020;58(12):1065-1075
DOI: https://doi.org/10.1007/s12275-020-0261-7
Published online: October 23, 2020

¹National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China, ²China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University, Beijing 100048, P. R. China, ³School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China, ⁴Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China¹National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China, ²China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology & Business University, Beijing 100048, P. R. China, ³School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China, ⁴Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, P. R. China

Corresponding author: Yu Deng , Tel: +86-510-85329031,

Received: 18 May 2020 • Revised: 27 July 2020 • Accepted: 12 August 2020

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Abstract

Adipic Acid (AA) is a valued platform chemical compound, which can be used as a precursor of nylon-6,6. Due to the generation of an enormous amount of nitric oxide metabolites and the growing depletion of oil resources as a result of AA production from a mixture of cyclohexanol and cyclohexanone, the microbial methods for synthesizing AA have attracted significant attention. Of the several AA-producing pathways, the reverse adipate degradation pathway in Thermobifida fusca (Tfu RADP) is reported to be the most efficient, which has been confirmed in Escherichia coli. In this study, the heterologous Tfu RADP was constructed for producing AA in S. cerevisiae by co-expressing genes of Tfu_ 0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576, and Tfu_ 2576. The AA titer combined with biomass, cofactors and other by-products was all determined after fermentation. During batch fermentation in a shake flask, the maximum AA titer was 3.83 mg/L, while the titer increased to 10.09 mg/L during fed-batch fermentation in a 5-L bioreactor after fermentation modification.

Keywords: Agricultural wastewater;Enhancement of bioreactor performance;Microbial N cycle;Nitrate pollution;Woodchip bioreactor

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Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae

J. Microbiol. 2020;58(12):1065-1075. Published online October 23, 2020

DOI: https://doi.org/10.1007/s12275-020-0261-7

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