Abstract
			
			Raman spectroscopy is a promising tool for identifying microbial
phenotypes based on single cell Raman spectra reflecting
cellular biochemical biomolecules. Recent studies
using Raman spectroscopy have mainly analyzed phenotypic
changes caused by microbial interactions or stress responses
(e.g., antibiotics) and evaluated the microbial activity or substrate
specificity under a given experimental condition using
stable isotopes. Lack of labelling and the nondestructive pretreatment
and measurement process of Raman spectroscopy
have also aided in the sorting of microbial cells with interesting
phenotypes for subsequently conducting physiology
experiments through cultivation or genome analysis. In this
review, we provide an overview of the principles, advantages,
and status of utilization of Raman spectroscopy for studies
linking microbial phenotypes and functions. We expect Raman
spectroscopy to become a next-generation phenotyping
tool that will greatly contribute in enhancing our understanding
of microbial functions in natural and engineered
systems.			
						
						
					 
		
		
		 
		
			Citations
			Citations to this article as recorded by  

- Intratumoral microbiome: implications for immune modulation and innovative therapeutic strategies in cancer
 Na Wang, Si Wu, Lanxiang Huang, Yue Hu, Xin He, Jourong He, Ben Hu, Yaqi Xu, Yuan Rong, Chunhui Yuan, Xiantao Zeng, Fubing Wang
 Journal of Biomedical Science.2025;[Epub]     CrossRef
- Rapid profiling of between-species metabolic interactions in cocultures by D2O‑probed single-cell Raman microspectroscopy
 Jianmei Wang, Lei Zhang, Xuechao Zhao, Jia Zhang, Rongze Chen, Yuetong Ji, Bo Ma, Yang Fang, Jian Xu
 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.2025; 343: 126513.     CrossRef
- Unsupervised classification of environmental marine microbes using a Raman spectra-based deep learning framework
 Fengyun Lü, Yanmei Zhang, Hongtao Liang, Luyang Sun
 Journal of Oceanology and Limnology.2025;[Epub]     CrossRef
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms
 Sandra Baaba Frempong, Markus Salbreiter, Sara Mostafapour, Aikaterini Pistiki, Thomas W. Bocklitz, Petra Rösch, Jürgen Popp
 Molecules.2024; 29(5): 1077.     CrossRef
- Advancing Soil Health: Challenges and Opportunities in Integrating Digital Imaging, Spectroscopy, and Machine Learning for Bioindicator Analysis
 Liang Wang, Ying Cheng, Islam Md Meftaul, Fang Luo, Muhammad Ashad Kabir, Richard Doyle, Zhenyu Lin, Ravi Naidu
 Analytical Chemistry.2024; 96(20): 8109.     CrossRef
- Phenotypic shifts induced by environmental pre-stressors modify antibiotic resistance in Staphylococcus aureus
 Gui Nam Wee, Eun Sun Lyou, Susmita Das Nishu, Tae Kwon Lee
 Frontiers in Microbiology.2023;[Epub]     CrossRef
- Formation and emergent dynamics of spatially organized microbial systems
 Kelsey Cremin, Sarah J. N. Duxbury, Jerko Rosko, Orkun S. Soyer
 Interface Focus.2023;[Epub]     CrossRef
- Demonstrating low Raman background in UV-written SiO2 waveguides
 Mathias Novik Jensen, James C. Gates, Alex I. Flint, Olav Gaute Hellesø
 Optics Express.2023; 31(19): 31092.     CrossRef
- Development of Polyhydroxybutyrate-Based Packaging Films and Methods to Their Ultrasonic Welding
 Viktoriia Talaniuk, Marcin Godzierz, Alina Vashchuk, Maksym Iurhenko, Paweł Chaber, Wanda Sikorska, Anastasiia Kobyliukh, Valeriy Demchenko, Sergiy Rogalsky, Urszula Szeluga, Grażyna Adamus
 Materials.2023; 16(20): 6617.     CrossRef
- Multi-element stable isotope Raman microspectroscopy of bacterial carotenoids unravels rare signal shift patterns and single-cell phenotypic heterogeneity
 Julian Weng, Kara Müller, Oleksii Morgaienko, Martin Elsner, Natalia P. Ivleva
 The Analyst.2023; 148(1): 128.     CrossRef
- Phenotypic convergence of bacterial adaption to sub-lethal antibiotic treatment
 Gui Nam Wee, Eun Sun Lyou, Jin-Kyung Hong, Jee Hyun No, Soo Bin Kim, Tae Kwon Lee
 Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
- Discrimination of Stressed and Non-Stressed Food-Related Bacteria Using Raman-Microspectroscopy
 Daniel Klein, René Breuch, Jessica Reinmüller, Carsten Engelhard, Peter Kaul
 Foods.2022; 11(10): 1506.     CrossRef
- Omics-based microbiome analysis in microbial ecology: from sequences to information
 Jang-Cheon Cho
 Journal of Microbiology.2021; 59(3): 229.     CrossRef
- Gold Nanomaterials as a Promising Integrated Tool for Diagnosis and Treatment of Pathogenic Infections—A Review
 Yan Zhou, Zefeng Wang, Yanling Peng, Feiying Wang, Le Deng
 Journal of Biomedical Nanotechnology.2021; 17(5): 744.     CrossRef
- Raman microspectroscopy for microbiology
 Kang Soo Lee, Zachary Landry, Fátima C. Pereira, Michael Wagner, David Berry, Wei E. Huang, Gordon T. Taylor, Janina Kneipp, Juergen Popp, Meng Zhang, Ji-Xin Cheng, Roman Stocker
 Nature Reviews Methods Primers.2021;[Epub]     CrossRef
- Flow cytometric monitoring of the bacterial phenotypic diversity in aquatic ecosystems
 Jin-Kyung Hong, Soo Bin Kim, Seok Hyun Ahn, Yongjoo Choi, Tae Kwon Lee
 Journal of Microbiology.2021; 59(10): 879.     CrossRef
- Roles of Phosphate Solubilizing Microorganisms from Managing Soil Phosphorus Deficiency to Mediating Biogeochemical P Cycle
 Jiang Tian, Fei Ge, Dayi Zhang, Songqiang Deng, Xingwang Liu
 Biology.2021; 10(2): 158.     CrossRef