The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner

Han Byeol Lee; Si Hyoung Park; Chang-Ro Lee

doi:10.1007/s12275-021-1130-8

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The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner: Han Byeol Lee , Si Hyoung Park , Chang-Ro Lee; Journal of Microbiology 2021;59(7):666-674.
DOI: https://doi.org/10.1007/s12275-021-1130-8
Published online: May 15, 2021

Department of Biological Sciences, Myongji University, Yongin 17058, Republic of KoreaDepartment of Biological Sciences, Myongji University, Yongin 17058, Republic of Korea

Corresponding author: Chang-Ro Lee , Tel: +82-31-330-6472;,

Received: 8 March 2021 • Revised: 8 April 2021 • Accepted: 8 April 2021

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Abstract

The inner membrane protein lipopolysaccharide assembly protein B (LapB) is an adaptor protein that activates the proteolysis of LpxC by an essential inner membrane metalloprotease, FtsH, leading to a decrease in the level of lipopolysaccharide in the membrane. In this study, we revealed the mechanism by which the essential inner membrane protein YejM regulates LapB and analyzed the role of the transmembrane domain of LapB in Escherichia coli. The transmembrane domain of YejM genetically and physically interacted with LapB and inhibited its function, which led to the accumulation of LpxC. The transmembrane domain of LapB was indispensable for both its physical interaction with YejM and its regulation of LpxC proteolysis. Notably, we found that the lapB mutant exhibited strong cold sensitivity and this phenotype was not associated with increased accumulation of LpxC. The transmembrane domain of LapB was also required for its role in adaptation to cold stress. Taken together, these
results
showed that LapB plays an important role in both the regulation of LpxC level, which is controlled by its interaction with the transmembrane domain of YejM, and adaptation to cold stress, which is independent of LpxC.

Keywords: Trypanosoma cruzi;autophagy;cadmium;mercury;nonessential metals;zinc

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The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner

J. Microbiol. 2021;59(7):666-674. Published online May 15, 2021

DOI: https://doi.org/10.1007/s12275-021-1130-8

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