Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains

Amanda Aparecida Seribelli; Tamara R. Machado Ribeiro; Patrick da Silva2†; Isabela Mancini Martins; Felipe Pinheiro Vilela; Marta I. Cazentini Medeiros; Kamila Chagas Peronni; Wilson Araújo da Silva Junior; Cristiano Gallina Moreira; Juliana Pfrimer Falcão

doi:10.1007/s12275-021-1082-z

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Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains: Amanda Aparecida Seribelli ^1†, Tamara R. Machado Ribeiro ^2†, Patrick da Silva2† , Isabela Mancini Martins ², Felipe Pinheiro Vilela ¹, Marta I. Cazentini Medeiros ³, Kamila Chagas Peronni ⁴, Wilson Araújo da Silva Junior ^4,5, Cristiano Gallina Moreira ², Juliana Pfrimer Falcão ¹; Journal of Microbiology 2021;59(9):861-870.
DOI: https://doi.org/10.1007/s12275-021-1082-z
Published online: August 12, 2021

¹School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo, Ribeirão Preto, São Paulo 05508-220, Brazil, ²Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo 01049-010, Brazil, ³Adolfo Lutz Institute - Ribeirão Preto, São Paulo 14085-410, Brazil, ⁴Regional Blood Center of the University Hospital of the Ribeirão Preto Medical School - University of São Paulo, Ribeirão Preto, São Paulo 05508-220, Brazil, ⁵Ribeirão Preto Medical School, Genetics Department - University of São Paulo, Ribeirão Preto, São Paulo 05508-220, Brazil¹School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo, Ribeirão Preto, São Paulo 05508-220, Brazil, ²Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo 01049-010, Brazil, ³Adolfo Lutz Institute - Ribeirão Preto, São Paulo 14085-410, Brazil, ⁴Regional Blood Center of the University Hospital of the Ribeirão Preto Medical School - University of São Paulo, Ribeirão Preto, São Paulo 05508-220, Brazil, ⁵Ribeirão Preto Medical School, Genetics Department - University of São Paulo, Ribeirão Preto, São Paulo 05508-220, Brazil

Corresponding author: Cristiano Gallina Moreira , Tel: +55-16-33154896,
Juliana Pfrimer Falcão , Tel: +55-16-33154896,

Received: 8 February 2021 • Revised: 12 May 2021 • Accepted: 6 July 2021

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Abstract

Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNAseq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37°C; (ii) compare it with the RNAseq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1β, IFN-γ, TNF-α, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis- related genes at 37°C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage.

Keywords: Comparative genomic analysis;Plant cell wall degrading enzymes;Stropharia rugosoannulata;Whole-genome sequencing

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Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains

J. Microbiol. 2021;59(9):861-870. Published online August 12, 2021

DOI: https://doi.org/10.1007/s12275-021-1082-z

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