Antiviral effects of human placenta hydrolysate (Laennec) against SARS-CoV-2 in vitro and in the ferret model

Eun-Ha Kim; Young-il Kim; Seung-Gyu Jang; Minju Im; Kyeongsoo Jeong; Young Ki Choi; Hae-Jung Han

doi:10.1007/s12275-021-1367-2

Articles

Page Path: HOME > J. Microbiol > Volume 59(11); 2021 > Article

Journal Article
Antiviral effects of human placenta hydrolysate (Laennec) against SARS-CoV-2 in vitro and in the ferret model: Eun-Ha Kim ^1,2, Young-il Kim ^1,2, Seung-Gyu Jang ^1,2, Minju Im ³, Kyeongsoo Jeong ³, Young Ki Choi ^1,2, Hae-Jung Han ³; Journal of Microbiology 2021;59(11):1056-1062.
DOI: https://doi.org/10.1007/s12275-021-1367-2
Published online: October 6, 2021

¹College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, Republic of Korea, ²Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju 28644, Republic of Korea , ³GREENCROSS WellBeing Co., Ltd., Seoul 07335, Republic of Korea¹College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, Republic of Korea, ²Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju 28644, Republic of Korea , ³GREENCROSS WellBeing Co., Ltd., Seoul 07335, Republic of Korea

Corresponding author: Young Ki Choi , Tel: +82-43-249-1617,
Hae-Jung Han , Tel: +82-43-249-1617,

Received: 6 July 2021 • Revised: 20 August 2021 • Accepted: 31 August 2021

prev next

50 Views
0 Download
6 Web of Science
7 Crossref
5 Scopus

Full Article

Abstract

The COVID-19 pandemic has caused unprecedented health, social, and economic crises worldwide. However, to date, there is an only a limited effective treatment for this disease. Human placenta hydrolysate (hPH) has previously been shown to be safe and to improve the health condition in patients with hyperferritinemia and COVID-19. In this study, we aimed to determine the antiviral effects of hPH against SARS-CoV-2 in vitro and in vivo models and compared with Remdesivir, an FDA-approved drug for COVID-19 treatment. To assess whether hPH inhibited SARS-CoV-2 replication, we determined the CC50, EC50, and selective index (SI) in Vero cells by infection with a SARS-CoV-2 at an MOI of 0.01. Further, groups of ferrets infected with 105.8 TCID50/ml of SARS-CoV-2 and treated with hPH at 2, 4, 6 dpi, and compared their clinical manifestation and virus titers in respiratory tracts with PBS control-treated group. The mRNA expression of immunerelated cytokines was determined by qRT-PCR. hPH treatment attenuated virus replication in a dose-dependent manner in vitro. In a ferret infection study, treatment with hPH resulted in minimal bodyweight loss and attenuated virus replication in the nasal wash, turbinates, and lungs of infected ferrets. In addition, qRT-PCR results revealed that the hPH treatment remarkably upregulated the gene expression of type I (IFN-α and IFN-β) and II (IFN-γ) IFNs in SARS-CoV-2 infected ferrets. Our data collectively suggest that hPH has antiviral efficacy against SARS-CoV-2 and might be a promising therapeutic agent for the treatment of SARS-CoV-2 infection.

Keywords: Aspergillus fumigatus;conidiation;ergosterol biosynthesis;metabolomics;transcriptomics

Figure & Data

References

Citations

Citations to this article as recorded by

Perinatal Hypoxia and Immune System Activation in Schizophrenia Pathogenesis: Critical Considerations During COVID-19 Pandemic
I Kawikova, K Hakenova, M Lebedeva, L Kleteckova, L Jakob, V Spicka, L Wen, F Spaniel, K Vales
Physiological Research.2024; : S615. CrossRef
Human Placenta Extract (HPH) Suppresses Inflammatory Responses in TNF-α/IFN-γ-Stimulated HaCaT Cells and a DNCB Atopic Dermatitis (AD)-Like Mouse Model
Jung Ok Lee, Youna Jang, A Yeon Park, Jung Min Lee, Kyeongsoo Jeong, So-Hyun Jeon, Hui Jin, Minju Im, Jae-Won Kim, Beom Joon Kim
Journal of Microbiology and Biotechnology.2024; 34(10): 1969. CrossRef
Systematic analysis of the pharmacology of standardized extracts of human placenta
T. E. Bogacheva, I. Yu. Torshin, O. A. Gromova
Pharmacokinetics and Pharmacodynamics.2024; (4): 3. CrossRef
Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef
Current state-of-the-art and potential future therapeutic drugs against COVID-19
Ailong Sha, Yi Liu, Haiyan Hao
Frontiers in Cell and Developmental Biology.2023;[Epub] CrossRef
SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets
Elizabeth E. Zumbrun, Samantha E. Zak, Eric D. Lee, Philip A. Bowling, Sara I. Ruiz, Xiankun Zeng, Jeffrey W. Koehler, Korey L. Delp, Russel R. Bakken, Shannon S. Hentschel, Holly A. Bloomfield, Keersten M. Ricks, Tamara L. Clements, April M. Babka, John
Viruses.2023; 15(12): 2341. CrossRef
Human placenta hydrolysates: from V.P. Filatov to the present day: Review
Olga A. Gromova, Ivan Yu. Torshin, Alexander G. Chuchalin, Valeriy А. Maximov
Terapevticheskii arkhiv.2022; 94(3): 434. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Antiviral effects of human placenta hydrolysate (Laennec) against SARS-CoV-2 in vitro and in the ferret model

J. Microbiol. 2021;59(11):1056-1062. Published online October 6, 2021

DOI: https://doi.org/10.1007/s12275-021-1367-2

Related articles