Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor

Yeonbum Kim; Jung-Hye Roe; Joo-Hong Park; Yong-Joon Cho; Kang-Lok Lee

doi:10.1007/s12275-021-1457-1

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Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor: Yeonbum Kim ¹, Jung-Hye Roe ¹, Joo-Hong Park ¹, Yong-Joon Cho ^1,2, Kang-Lok Lee ³; Journal of Microbiology 2021;59(12):1083-1091.
DOI: https://doi.org/10.1007/s12275-021-1457-1
Published online: December 4, 2021

¹Department of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea, ²The Research Institute of Basic Sciences, Seoul National University, Seoul 08826, Republic of Korea, ³Department of Biology Education, IALS, Gyeongsang National University, Jinju 52828, Republic of Korea¹Department of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea, ²The Research Institute of Basic Sciences, Seoul National University, Seoul 08826, Republic of Korea, ³Department of Biology Education, IALS, Gyeongsang National University, Jinju 52828, Republic of Korea

Corresponding author: Yong-Joon Cho ,
Kang-Lok Lee ,

Received: 6 September 2021 • Revised: 8 October 2021 • Accepted: 20 October 2021

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Abstract

CatR, a peroxide-sensing transcriptional repressor of Fur family, can de-repress the transcription of the catA gene encoding catalase upon peroxide stress in Streptomyces coelicolor. Since CatR-regulated genes other than catA and its own gene catR have not been identified in detail, the understanding of the role of CatR regulon is very limited. In this study, we performed transcriptomic analysis to identify genes influenced by both 􀈟􀂊atR mutation and hydrogen peroxide treatment. Through ChIP-qPCR and other analyses, a new consensus sequence was found in CatR-responsive promoter region of catR gene and catA operon for direct regulation. In addition, vtlA (SCO2027) and SCO4983 were identified as new members of the CatR regulon. Expression levels of iron uptake genes were reduced by hydrogen peroxide and a DmdR1 binding sequence was identified in promoters of these genes. The increase in free iron by hydrogen peroxide was thought to suppress the iron import system by DmdR1. A putative exporter protein VtlA regulated by CatR appeared to reduce intracellular iron to prevent oxidative stress. The name vtlA (VIT1-like transporter) was proposed for iron homeostasis related gene SCO2027.

Keywords: Flavobacterium;Flavobacterium panacagri;Flavobacterium psychrotrophum;Genome;Novel species

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Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor

J. Microbiol. 2021;59(12):1083-1091. Published online December 4, 2021

DOI: https://doi.org/10.1007/s12275-021-1457-1

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