Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae

Alex Wegner; Florencia Casanova; Marco Loehrer; Angelina Jordine; Stefan Bohnert; Xinyu Liu; Zhengguang Zhang; Ulrich Schaffrath

doi:10.1007/s12275-022-1074-7

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Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae: Alex Wegner ^1†, Florencia Casanova ^1†, Marco Loehrer ¹, Angelina Jordine ¹, Stefan Bohnert ¹, Xinyu Liu ², Zhengguang Zhang ², Ulrich Schaffrath ¹; Journal of Microbiology 2022;60(1):79-88.
DOI: https://doi.org/10.1007/s12275-022-1074-7
Published online: December 29, 2021

¹Department of Plant Physiology, RWTH Aachen University, Aachen 52056, Germany, ²Department of Plant Pathology, Nanjing Agricultural University, Jiangsu 210095, P. R. China¹Department of Plant Physiology, RWTH Aachen University, Aachen 52056, Germany, ²Department of Plant Pathology, Nanjing Agricultural University, Jiangsu 210095, P. R. China

Corresponding author: Ulrich Schaffrath , Tel: +49-241-8020100,

Received: 4 February 2021 • Revised: 20 August 2021 • Accepted: 27 September 2021

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Abstract

Phytopathogenic fungi are known to secrete specific proteins which act as virulence factors and promote host colonization. Some of them are enzymes with plant cell wall degradation capability, like pectate lyases (Pls). In this work, we examined the involvement of Pls in the infection process of Magnaporthe oryzae, the causal agent of rice blast disease. From three Plgenes annotated in the M. oryzae genome, only transcripts of MoPL1 considerably accumulated during the infection process with a peak at 72 h post inoculation. Both, gene deletion and a constitutive expression of MoPL1 in M. oryzae led to a significant reduction in virulence. By contrast, mutants that constitutively expressed an enzymatic inactive version of MoPl1 did not differ in virulence compared to the wild type isolate. This indicates that the enzymatic activity of MoPl1 is responsible for diminished virulence, which is presumably due to degradation products recognized as danger associated molecular patterns (DAMPs), which strengthen the plant immune response. Microscopic analysis of infection sites pointed to an increased plant defense response. Additionally, MoPl1 tagged with mRFP, and not the enzymatic inactive version, focally accumulated in attacked plant cells beneath appressoria and at sites where fungal hyphae transverse from one to another cell. These findings shed new light on the role of pectate lyases during tissue colonization in the necrotrophic stage of M. oryzae's life cycle.

Keywords: Myxococcus xanthus;encapsulin;myxobacteria;phase variation

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Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae

J. Microbiol. 2022;60(1):79-88. Published online December 29, 2021

DOI: https://doi.org/10.1007/s12275-022-1074-7

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