Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection

Hongdong Liao; Xiangyu Wen; Xuelei Deng; Yonghong Wu; Jianping Xu; Xin Li; Shudong Zhou; Xuefeng Li; Chunhui Zhu; Feng Luo; Yanqing Ma; Jingyuan Zheng

doi:10.1007/s12275-022-1603-4

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Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection: Hongdong Liao ¹, Xiangyu Wen ¹, Xuelei Deng ¹, Yonghong Wu ^2,3, Jianping Xu ^4,5, Xin Li ¹, Shudong Zhou ², Xuefeng Li ², Chunhui Zhu ⁶, Feng Luo ⁷, Yanqing Ma ^2,3, Jingyuan Zheng ^1,2,3; Journal of Microbiology 2022;60(5):511-525.
DOI: https://doi.org/10.1007/s12275-022-1603-4
Published online: March 31, 2022

¹College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P. R. China, ²Institute of Vegetables, Hunan Academy of Agricultural Sciences, Changsha 410125, P. R. China, ³Longping Branch, Graduate School of Hunan University, Changsha 410125, P. R. China, ⁴Institute of Bast Fiber Crops and Center for Southern Economic Crops, Chinese Academy of Agricultural Science, Changsha 410205, P. R. China, ⁵Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada, ⁶Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, P. R. China, ⁷Sanya Science & Technology Academy of Hainan National Breeding and Multiplication, Sanya, Hainan 572000, P. R. China¹College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha 410082, P. R. China, ²Institute of Vegetables, Hunan Academy of Agricultural Sciences, Changsha 410125, P. R. China, ³Longping Branch, Graduate School of Hunan University, Changsha 410125, P. R. China, ⁴Institute of Bast Fiber Crops and Center for Southern Economic Crops, Chinese Academy of Agricultural Science, Changsha 410205, P. R. China, ⁵Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada, ⁶Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, P. R. China, ⁷Sanya Science & Technology Academy of Hainan National Breeding and Multiplication, Sanya, Hainan 572000, P. R. China

Corresponding author: Yanqing Ma ,
Jingyuan Zheng ,

Received: 19 November 2021 • Revised: 26 January 2022 • Accepted: 4 February 2022

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Abstract

Infection by Sclerotium rolfsii will cause serious disease and lead to significant economic losses in chili pepper. In this study, the response of pepper during S. rolfsii infection was explored by electron microscopy, physiological determination and integrated proteome and metabolome analyses. Our results showed that the stomata of pepper stems were important portals for S. rolfsii infection. The plant cell morphology was significantly changed at the time of the fungal hyphae just contacting (T1) or surrounding (T2) the pepper. The chlorophyll, carotenoid, and MDA contents and the activities of POD, SOD, and CAT were markedly upregulated at T1 and T2. Approximately 4129 proteins and 823 metabolites were clearly identified in proteome and metabolome analyses, respectively. A change in 396 proteins and 54 metabolites in pepper stem tissues was observed at T1 compared with 438 proteins and 53 metabolites at T2. The proteins and metabolites related to photosynthesis and antioxidant systems in chloroplasts and mitochondria were disproportionally affected by S. rolfsii infection, impacting carbohydrate and amino acid metabolism. This study provided new insights into the response mechanism in pepper stems during S. rolfsii infection, which can guide future work on fungal disease resistance breeding in pepper.

Keywords: Bacteroidetes;Mucilaginibacter limnophilus sp. nov.;Sphingobacteriaceae;Sphingobacteriales;Sphingobacteriia;polyphasic taxonomy

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Integrated proteomic and metabolomic analyses reveal significant changes in chloroplasts and mitochondria of pepper (Capsicum annuum L.) during Sclerotium rolfsii infection

J. Microbiol. 2022;60(5):511-525. Published online March 31, 2022

DOI: https://doi.org/10.1007/s12275-022-1603-4

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