Prevalence and characteristics of the mcr-1 gene in retail meat samples in Zhejiang Province, China

Biao Tang; Jiang Chang; Yi Luo; Han Jiang; Canying Liu; Xingning Xiao; Xiaofeng Ji; Hua Yang

doi:10.1007/s12275-022-1597-y

Articles

Page Path: HOME > J. Microbiol > Volume 60(6); 2022 > Article

Journal Article
Prevalence and characteristics of the mcr-1 gene in retail meat samples in Zhejiang Province, China: Biao Tang ¹, Jiang Chang ², Yi Luo ³, Han Jiang ⁴, Canying Liu ⁵, Xingning Xiao ¹, Xiaofeng Ji ¹, Hua Yang ¹; Journal of Microbiology 2022;60(6):610-619.
DOI: https://doi.org/10.1007/s12275-022-1597-y
Published online: March 31, 2022

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310004, P. R. China, ²School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 200240, P. R. China, ³Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P. R. China, ⁴Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, China Jiliang University, Hangzhou, Zhejiang 310018, P. R. China, ⁵School of Life Science and Engineering, Foshan University, Foshan, Guangdong 528225, P. R. China¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310004, P. R. China, ²School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 200240, P. R. China, ³Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, P. R. China, ⁴Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, China Jiliang University, Hangzhou, Zhejiang 310018, P. R. China, ⁵School of Life Science and Engineering, Foshan University, Foshan, Guangdong 528225, P. R. China

Corresponding author: Biao Tang ,
Hua Yang ,

Received: 15 November 2021 • Revised: 21 January 2022 • Accepted: 14 February 2022

prev next

53 Views
0 Download
10 Web of Science
8 Crossref
10 Scopus

Full Article

Abstract

Considering the serious threat to food safety and public health posed by pathogens with colistin resistance, colistin was banned as a growth promoter in 2017 in China. In recent years, the resistance rate of Escherichia coli isolated from animal intestines or feces to colistin has decreased. However, the prevalence and characteristics of the mcr-1 gene in retail meat have not been well explored. Herein, 106 mcr-1-negative and 16 mcr- 1-positive E. coli isolates were randomly recovered from 120 retail meat samples and screened using colistin. The 106 E. coli isolates showed maximum resistance to sulfafurazole (73.58%) and tetracycline (62.26%) but susceptibility to colistin (0.00%). All 16 mcr-1-positive E. coli isolates showed resistance to colistin, were extended spectrum beta-lactamase (ESBL)-positive and exhibited complex multidrug resistance (MDR). For these 16 isolates, 17 plasmid replicons and 42 antibiotic resistance genes were identified, and at least 7 antibiotic resistance genes were found in each isolate. Acquired disinfectant resistance genes were identified in 75.00% (12/16) of the isolates. Furthermore, comparative genomic and phylogenetic analysis
results
indicated that these 16 mcr-1-positive E. coli isolates and the most prevalent mcr-1-harboring IncI2 plasmid in this study were closely related to other previously reported mcr-1-positive E. coli isolates and the IncI2 plasmid, respectively, showing their wide distribution. Taken together, our findings showed that retail meat products were a crucial reservoir of mcr-1 during the colistin ban period and should be continuously monitored.

Keywords: Edaphic properties;Elevation;Semiarid environment;Soil bacterial diversity;Xinglong Mountains

Figure & Data

References

Citations

Citations to this article as recorded by

Regression models from portable NIR spectra for predicting the carcass traits and meat quality of beef cattle
Nathália Veloso Trópia, Rizielly Saraiva Reis Vilela, Flávia Adriane de Sales Silva, Dhones Rodrigues Andrade, Adailton Camêlo Costa, Fernando Alerrandro Andrade Cidrini, Jardeson de Souza Pinheiro, Pauliane Pucetti, Mario Luiz Chizzotti, Sebastião de Cam
PLOS ONE.2024; 19(5): e0303946. CrossRef
IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine
Jiangang Ma, Juan Wang, Hua Yang, Mengru Su, Ruichao Li, Li Bai, Jie Feng, Yuting Huang, Zengqi Yang, Biao Tang
Frontiers in Microbiology.2023;[Epub] CrossRef
Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching hospital
Chenlu Xiao, Xuming Li, Lianjiang Huang, Huiluo Cao, Lizhong Han, Yuxing Ni, Han Xia, Zhitao Yang
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Farm to table: colistin resistance hitchhiking through food
Absar Talat, Carla Miranda, Patrícia Poeta, Asad U. Khan
Archives of Microbiology.2023;[Epub] CrossRef
Detection of mcr-1-harbouring Escherichia coli by quantum dot labelling of synthetic small peptides mimicking lipopolysaccharide receptors
Chenghao Wang, Biao Tang, Jiusheng Wu, Xi Jin, Shuwen Ke, Hua Yang, Yuehuan Liu
International Journal of Antimicrobial Agents.2023; 62(3): 106898. CrossRef
Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China
Biao Tang, Juan Ni, Jiahui Lin, Yangying Sun, Hui Lin, Yuehong Wu, Hua Yang, Min Yue
Frontiers in Microbiology.2022;[Epub] CrossRef
Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China
Wei Zhou, Rumeng Lin, Zhijin Zhou, Jiangang Ma, Hui Lin, Xue Zheng, Jingge Wang, Jing Wu, Yuzhi Dong, Han Jiang, Hua Yang, Zhangnv Yang, Biao Tang, Min Yue
Frontiers in Microbiology.2022;[Epub] CrossRef
The Occurrence and Genomic Characteristics of mcr-1-Harboring Salmonella from Retail Meats and Eggs in Qingdao, China
Changan Li, Xiulei Gu, Liping Zhang, Yuqing Liu, Yan Li, Ming Zou, Baotao Liu
Foods.2022; 11(23): 3854. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Prevalence and characteristics of the mcr-1 gene in retail meat samples in Zhejiang Province, China

J. Microbiol. 2022;60(6):610-619. Published online March 31, 2022

DOI: https://doi.org/10.1007/s12275-022-1597-y

Related articles