Early gut microbiota in very low and extremely low birth weight preterm infants with feeding intolerance: a prospective case-control study

Ling Liu; Dang Ao; Xiangsheng Cai; Peiyi Huang; Nali Cai; Shaozhu Lin; Benqing Wu

doi:10.1007/s12275-022-2180-2

Articles

Page Path: HOME > J. Microbiol > Volume 60(10); 2022 > Article

Observational Study
Early gut microbiota in very low and extremely low birth weight preterm infants with feeding intolerance: a prospective case-control study: Ling Liu ^1,2, Dang Ao ², Xiangsheng Cai ³, Peiyi Huang ², Nali Cai ², Shaozhu Lin ², Benqing Wu ^1,3; Journal of Microbiology 2022;60(10):1021-1031.
DOI: https://doi.org/10.1007/s12275-022-2180-2
Published online: August 19, 2022

¹Jinan University, Guangzhou, Guangdong 510000, P. R. China, ²Department of Pediatrics, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524000, P. R. China, ³University of the Chinese Academy of Sciences - Shenzhen Hospital, Shenzhen, Guangdong 518000, P. R. China¹Jinan University, Guangzhou, Guangdong 510000, P. R. China, ²Department of Pediatrics, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524000, P. R. China, ³University of the Chinese Academy of Sciences - Shenzhen Hospital, Shenzhen, Guangdong 518000, P. R. China

Corresponding author: Benqing Wu ,

Received: 25 April 2022 • Revised: 30 June 2022 • Accepted: 7 July 2022

prev next

57 Views
0 Download
12 Web of Science
10 Crossref
8 Scopus

Full Article

Abstract

The potential role of the gut microbiota in the pathogenesis of feeding intolerance (FI) remains unclear. Understanding the role of the gut microbiota could provide a new avenue for microbiota-targeted therapeutics. This study aimed to explore the associations between aberrant gut microbiota and FI in very low or extremely low birth weight (VLBW/ELBW) preterm infants. In this observational case-control study, VLBW/ ELBW infants were divided into two groups: FI group and feeding tolerance (FT) group. 16S rRNA gene sequencing was performed to analyze the gut microbial diversity and composition of the infants. The differences in the gut microbiota of the two groups were compared. In total, 165 stool samples were obtained from 44 infants, among which, 31 developed FI and 13 served as controls. Alpha diversity was the highest in the meconium samples of the two groups. LEfSe analysis revealed that the abundances of Peptostreptococcaceae, Clostridiales and Clostridia in the FT group were significantly higher than in the FI group. At the phylum level, the FI group was dominated by Proteobacteria, and the FT group was dominated by Firmicutes. The meconium samples of the FI group had higher proportions of γ-proteobacteria and Escherichia-Shigella and a lower proportion of Bacteroides compared with the FT group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that aberrant gut bacteria in the FI group were strongly associated with dysregulation of C5-Brancheddibasic- acid-metabolism, protein kinases, and sporulation. These findings reveal candidate microbial markers to prevent FI. Increased relative abundances of γ-proteobacteria and Escherichia-Shigella and decreased abundance of Bacteroides in meconium were associated with an increased risk of FI, while Peptostreptococcaceae, Clostridiales and Clostridia reduced the risk of FI in VLBW/ELBW infants.

Keywords: Helicobacter pylori;geographic diversity;oipA;outer membrane protein

Figure & Data

References

Citations

Citations to this article as recorded by

Reduced Gut Bacterial Diversity in Early Life Predicts Feeding Intolerance in Preterm Neonates
Maria Di Chiara, Alessandro Lazzaro, Daniela Scribano, Maria Trancassini, Valeria Pietropaolo, Michele Sonnessa, Chiara De Luca, Rita Prota, Elisa Onestà, Gianluigi Laccetta, Gianluca Terrin
Tropical Medicine and Infectious Disease.2024; 9(8): 174. CrossRef
Calorie restriction during gestation impacts maternal and offspring fecal microbiome in mice
Stephanie P. Gilley, Meghan L. Ruebel, Sree V. Chintapalli, Clyde J. Wright, Paul J. Rozance, Kartik Shankar
Frontiers in Endocrinology.2024;[Epub] CrossRef
Dynamics alteration of the gut microbiota and faecal metabolomes in very low or extremely low birth weight infants: a Chinese single-center, prospective cohort study
Ling Liu, Chaohong Chen, YeShan Li, Dang Ao, Jiayuan Wu, Nali Cai, Wen Li, Min Xiang
Frontiers in Microbiology.2024;[Epub] CrossRef
Metabolic and fecal microbial changes in adult fetal growth restricted mice
Stephanie P. Gilley, Miguel A. Zarate, Lijun Zheng, Purevsuren Jambal, Deaunabah N. Yazza, Sree V. Chintapalli, Paul S. MacLean, Clyde J. Wright, Paul J. Rozance, Kartik Shankar
Pediatric Research.2024; 95(3): 647. CrossRef
A digital twin of the infant microbiome to predict neurodevelopmental deficits
Nicholas Sizemore, Kaitlyn Oliphant, Ruolin Zheng, Camilia R. Martin, Erika C. Claud, Ishanu Chattopadhyay
Science Advances.2024;[Epub] CrossRef
Investigating prenatal and perinatal factors on meconium microbiota: a systematic review and cohort study
Jenni Turunen, Mysore V. Tejesvi, Niko Paalanne, Tytti Pokka, Sajeen Bahadur Amatya, Surbhi Mishra, Anna Kaisanlahti, Justus Reunanen, Terhi Tapiainen
Pediatric Research.2024; 95(1): 135. CrossRef
Novel scoring system for early diagnosis of necrotizing enterocolitis: integrating clinical and laboratory data with urinary caveolin-1 levels
Brigitta I.R.V. Corebima, Rinawati Rohsiswatmo, Dewi Santosaningsih, Wisnu Barlianto, Kusworini Handono
Archives of Medical Science.2023; 20(2): 444. CrossRef
Dynamics and Crosstalk between Gut Microbiota, Metabolome, and Fecal Calprotectin in Very Preterm Infants: Insights into Feeding Intolerance
Luyang Hong, Yihuang Huang, Junyan Han, Shujuan Li, Lan Zhang, Siyuan Jiang, Qi Zhou, Xincheng Cao, Weiyin Yu, Yi Yang, Shangyu Hong, Yufeng Zhou, Weili Yan, Yun Cao
Nutrients.2023; 15(22): 4849. CrossRef
Characteristics of Gut Microbiota in Small for Gestational Age Infants with Very Low Birth Weight
Hung-Yang Chang, Jen-Shiu Chiang Chiau, Jui-Hsing Chang, Chyong-Hsin Hsu, Chia-Ying Lin, Mary Hsin-Ju Ko, Hung-Chang Lee
Nutrients.2022; 14(23): 5158. CrossRef
Compositional Differences of Meconium Microbiomes of Preterm and Term Infants, and Infants That Developed Necrotizing Enterocolitis or Feeding Intolerance
Hyun Mi Kang, Sol Kim, Seok Hwang-Bo, In Hyuk Yoo, Yu-Mi Seo, Moon Yeon Oh, Soo-Ah Im, Young-Ah Youn
Pathogens.2022; 12(1): 55. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Early gut microbiota in very low and extremely low birth weight preterm infants with feeding intolerance: a prospective case-control study

J. Microbiol. 2022;60(10):1021-1031. Published online August 19, 2022

DOI: https://doi.org/10.1007/s12275-022-2180-2

Related articles