Characteristic alterations of gut microbiota in uncontrolled gout

Asad ul-Haq; Kyung-Ann Lee; Hoonhee Seo; Sukyung Kim; Sujin Jo; Kyung Min Ko; Su-Jin Moon; Yun Sung Kim; Jung Ran Choi; Ho-Yeon Song; Hyun-Sook Kim

doi:10.1007/s12275-022-2416-1

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Characteristic alterations of gut microbiota in uncontrolled gout: Asad ul-Haq ^1,2, Kyung-Ann Lee ¹, Hoonhee Seo ^2,3, Sukyung Kim ³, Sujin Jo ², Kyung Min Ko ⁴, Su-Jin Moon ⁵, Yun Sung Kim ⁶, Jung Ran Choi ⁷, Ho-Yeon Song ^2,3, Hyun-Sook Kim ¹; Journal of Microbiology 2022;60(12):1178-1190
DOI: https://doi.org/10.1007/s12275-022-2416-1
Published online: November 24, 2022

¹Division of Rheumatology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul 04401, Republic of Korea, ²Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan 31538, Republic of Korea, ³Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 33151, Republic of Korea, ⁴Division of Rheumatology, Department of Internal Medicine, International St. Mary’s Hospital, Catholic Kwandong University College of Medicine, Incheon 22711, Republic of Korea, ⁵Division of Rheumatology, Department of Internal Medicine, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 07345, Republic of Korea, ⁶Division of Rheumatology, Department of Internal Medicine, Chosun University College of Medicine, Gwangju 61452, Republic of Korea, ⁷Divison of Rheumatology, Department of Internal Medicine, Pohang St. Mary’s Hospital, Pohang 37661, Republic of Korea¹Division of Rheumatology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul 04401, Republic of Korea, ²Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan 31538, Republic of Korea, ³Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 33151, Republic of Korea, ⁴Division of Rheumatology, Department of Internal Medicine, International St. Mary’s Hospital, Catholic Kwandong University College of Medicine, Incheon 22711, Republic of Korea, ⁵Division of Rheumatology, Department of Internal Medicine, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 07345, Republic of Korea, ⁶Division of Rheumatology, Department of Internal Medicine, Chosun University College of Medicine, Gwangju 61452, Republic of Korea, ⁷Divison of Rheumatology, Department of Internal Medicine, Pohang St. Mary’s Hospital, Pohang 37661, Republic of Korea

Corresponding author: Hyun-Sook Kim , Tel: +82-2-710-3214,

Received: 13 September 2022 • Revised: 9 November 2022 • Accepted: 15 November 2022

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Abstract

Microbiome research has been on the rise recently for a more in-depth understanding of gout. Meanwhile, there is a need to understand the gut microbiome related to uric acid-lowering drug resistance. In this study, 16S rRNA gene-based microbiota analysis was performed for a total of 65 stool samples from 17 healthy controls and 48 febuxostat-treated gout patients (including 28 controlled subjects with decreased uric acid levels and 20 uncontrolled subjects with non-reduced uric acid levels). Alpha diversity of bacterial community decreased in the healthy control, controlled, and uncontrolled groups. In the case of beta diversity, the bacterial community was significantly different among groups (healthy control, controlled, and uncontrolled groups). Taxonomic biomarker analysis revealed the increased population of g-Bifidobacterium in healthy controls and g-Prevotella in uncontrolled patients. PCR further confirmed this result at the species level. Additionally, functional metagenomics predictions led to the exploration of various functional biomarkers, including purine metabolism. The results of this study can serve as a basis for developing potential new strategies for diagnosing and treating gout from microbiome prospects.

Keywords: bioinformatics;environmental DNA;metagenomics;microbiome;shotgun metagenomic sequencing

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Characteristic alterations of gut microbiota in uncontrolled gout

J. Microbiol. 2022;60(12):1178-1190. Published online November 24, 2022

DOI: https://doi.org/10.1007/s12275-022-2416-1

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