Recent Advances in CRISPR‑Cas Technologies for Synthetic Biology

Song Hee Jeong; Ho Joung Lee; Sang Jun Lee

doi:10.1007/s12275-022-00005-5

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Recent Advances in CRISPR‑Cas Technologies for Synthetic Biology: Song Hee Jeong , Ho Joung Lee , Sang Jun Lee; Journal of Microbiology 2023;61(1):13-36.
DOI: https://doi.org/10.1007/s12275-022-00005-5
Published online: February 1, 2023

Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of KoreaDepartment of Systems Biotechnology, Chung-Ang University, Anseong 17546, Republic of Korea

Corresponding author: Sang Jun Lee ,

Received: 11 October 2022 • Revised: 15 November 2022 • Accepted: 15 November 2022

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Abstract

With developments in synthetic biology, “engineering biology” has emerged through standardization and platformization based on hierarchical, orthogonal, and modularized biological systems. Genome engineering is necessary to manufacture and design synthetic cells with desired functions by using bioparts obtained from sequence databases. Among various tools, the CRISPR-Cas system is modularly composed of guide RNA and Cas nuclease; therefore, it is convenient for editing the genome freely. Recently, various strategies have been developed to accurately edit the genome at a single nucleotide level. Furthermore, CRISPR-Cas technology has been extended to molecular diagnostics for nucleic acids and detection of pathogens, including disease-causing viruses. Moreover, CRISPR technology, which can precisely control the expression of specific genes in cells, is evolving to find the target of metabolic biotechnology. In this review, we summarize the status of various CRISPR technologies that can be applied to synthetic biology and discuss the development of synthetic biology combined with CRISPR technology in microbiology.

Keywords: Salicibibacter halophilus;alkalitolerant;bacterium;halophile;kimchi;taxonomy

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Recent Advances in CRISPR‑Cas Technologies for Synthetic Biology

J. Microbiol. 2023;61(1):13-36. Published online February 1, 2023

DOI: https://doi.org/10.1007/s12275-022-00005-5

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