Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments

Yuyuan Huang; Yifan Jiao; Sihui Zhang; Yuanmeihui Tao; Suping Zhang; Dong Jin; Ji Pu; Liyun Liu; Jing Yang; Shan Lu

doi:10.1007/s12275-023-00029-5

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Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments: Yuyuan Huang ¹, Yifan Jiao ², Sihui Zhang ^1,3, Yuanmeihui Tao ¹, Suping Zhang ^1,4, Dong Jin ¹, Ji Pu ¹, Liyun Liu ¹, Jing Yang ^1,5, Shan Lu ^1,5; Journal of Microbiology 2023;61(4):379-388.
DOI: https://doi.org/10.1007/s12275-023-00029-5
Published online: March 16, 2023

¹State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing 102206, People’s Republic of China, ²Taiyuan Central Hospital of Shanxi Medical University, Taiyuan 030009, People’s Republic of China, ³Peking University School of Public Health, Beijing 100083, People’s Republic of China, ⁴Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, People’s Republic of China, ⁵Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, People’s Republic of China¹State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing 102206, People’s Republic of China, ²Taiyuan Central Hospital of Shanxi Medical University, Taiyuan 030009, People’s Republic of China, ³Peking University School of Public Health, Beijing 100083, People’s Republic of China, ⁴Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, People’s Republic of China, ⁵Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, People’s Republic of China

Corresponding author: Jing Yang ,
Shan Lu ,

Received: 14 December 2022 • Revised: 8 February 2023 • Accepted: 13 February 2023

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Abstract

Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E) in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5–98.6%) and O. murale 01-Gi-040T (98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin ( C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).

Keywords: Acidithiobacillus ferrooxidans;E121D mutation;MD simulation;RCY/Cyc1 complex;respiratory chain

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Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments

J. Microbiol. 2023;61(4):379-388. Published online March 16, 2023

DOI: https://doi.org/10.1007/s12275-023-00029-5

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