Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status

Yingying Ma; Yanpeng Li; Yanmei Liu; Le Cao; Xiao Han; Shujun Gao; Chiyu Zhang

doi:10.1007/s12275-023-00039-3

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Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status: Yingying Ma ¹, Yanpeng Li ¹, Yanmei Liu ^2,3, Le Cao ¹, Xiao Han ^2,3, Shujun Gao ^2,3, Chiyu Zhang ¹; Journal of Microbiology 2023;61(4):423-432.
DOI: https://doi.org/10.1007/s12275-023-00039-3
Published online: April 3, 2023

¹Shanghai Public Health Clinical Center, Fudan University, Shanghai 200437, People’s Republic of China, ²Center of Diagnosis and Treatment for Cervical and Uterine Cavity Disease, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, People’s Republic of China, ³Shanghai Key Laboratory of Female Reproductive Endocrine‑Related Disease, Fudan University, Shanghai 200011, People’s Republic of China¹Shanghai Public Health Clinical Center, Fudan University, Shanghai 200437, People’s Republic of China, ²Center of Diagnosis and Treatment for Cervical and Uterine Cavity Disease, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, People’s Republic of China, ³Shanghai Key Laboratory of Female Reproductive Endocrine‑Related Disease, Fudan University, Shanghai 200011, People’s Republic of China

Corresponding author: Shujun Gao ,
Chiyu Zhang ,

Received: 4 January 2023 • Revised: 20 February 2023 • Accepted: 6 March 2023

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Abstract

Vaginal microbiome composition was demonstrated to be associated with cervical disease. The colonization characteristics of vaginal microbes and their association with the different cervical disease status, especially cervical cancer (CC), are rarely investigated. In this cross-sectional study, we characterized the vaginal microbiome of women with different status of cervical diseases, including 22 NV + (normal tissue with HPV infection), low-grade squamous intraepithelial lesion (LSIL, n = 45), high-grade squamous intraepithelial lesion (HSIL, n = 36) and CC (n = 27) using bacterial 16S DNA sequencing. Thirty HPV-negative women with normal tissue were used as the control group. We found that higher diversity of microbiome with gradual depletion of Lactobacillus, especially L. crispatus, was associated with the severity of cervical disease. High-risk HPV16 infection was associated with higher microbiome diversity and depletion of Lactobacillus in high-grade cervical diseases (i.e. HSIL and CC). The CC group was characterized by higher levels of Fannyhessea vaginae, Prevotella, Bacteroides, Finegoldia, Vibrio, Veillonella, Peptostreptococcus, and Dialister. Co-occurrence network analyses showed that negative correlations were exclusively observed between Lactobacillus and other bacteria, and almost all non-Lactobacillus bacteria were positively correlated with each other. In particular, the most diverse and complex co-occurrence network of vaginal bacteria, as well as a complete loss of L. crispatus, was observed in women with CC. Logistic regression model identified HPV16 and Lactobacillus as significant risk and protective factors for CC, respectively. These results suggest that specific Lactobacillus species (e.g. L. crispatus and L. iners) can be used as important markers to target prevention measures prioritizing HPV16-infected women and other hrHPV-infected women for test, vaccination and treat initiatives.

Keywords: H. pylori;PI3K/AKT;energy metabolism;gamma-glutamyltransferase;mesenchymal stem cell;methylation

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Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status

J. Microbiol. 2023;61(4):423-432. Published online April 3, 2023

DOI: https://doi.org/10.1007/s12275-023-00039-3

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