Heterologous Production and Structure Determination of a New Lanthipeptide Sinosporapeptin Using a Cryptic Gene Cluster in an Actinobacterium Sinosporangium siamense

Keita Saito; Keiichiro Mukai; Issara Kaweewan; Hiroyuki Nakagawa; Takeshi Hosaka; Shinya Kodani

doi:10.1007/s12275-023-00059-z

Articles

Page Path: HOME > J. Microbiol > Volume 61(6); 2023 > Article

Journal Article
Heterologous Production and Structure Determination of a New Lanthipeptide Sinosporapeptin Using a Cryptic Gene Cluster in an Actinobacterium Sinosporangium siamense: Keita Saito ¹, Keiichiro Mukai ^2,3, Issara Kaweewan ⁴, Hiroyuki Nakagawa ⁵, Takeshi Hosaka ^2,3, Shinya Kodani ^1,4,6; Journal of Microbiology 2023;61(6):641-648.
DOI: https://doi.org/10.1007/s12275-023-00059-z
Published online: June 12, 2023

¹Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422‑8529, Japan, ²Graduate School of Medicine, Science and Technology, Shinshu University, Nagano 399‑4598, Japan, ³Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano 399‑4598, Japan, ⁴Faculty of Agriculture, Shizuoka University, Shizuoka 422‑8529, Japan, ⁵Research Center for Advanced Analysis, Core Technology Research Headquarters, National Agriculture and Food Research Organization (NARO), Ibaraki 305‑8642, Japan, ⁶College of Agriculture, Academic Institute, Shizuoka University, Shizuoka 422‑8529, Japan¹Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422‑8529, Japan, ²Graduate School of Medicine, Science and Technology, Shinshu University, Nagano 399‑4598, Japan, ³Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano 399‑4598, Japan, ⁴Faculty of Agriculture, Shizuoka University, Shizuoka 422‑8529, Japan, ⁵Research Center for Advanced Analysis, Core Technology Research Headquarters, National Agriculture and Food Research Organization (NARO), Ibaraki 305‑8642, Japan, ⁶College of Agriculture, Academic Institute, Shizuoka University, Shizuoka 422‑8529, Japan

Corresponding author: Shinya Kodani ,

Received: 2 February 2023 • Revised: 23 April 2023 • Accepted: 8 May 2023

prev next

56 Views
0 Download
5 Web of Science
3 Crossref
4 Scopus

Full Article

Abstract

Lipolanthine is a subclass of lanthipeptide that has the modification of lipid moiety at the N-terminus. A cryptic biosynthetic gene cluster comprising four genes (sinA, sinKC, sinD, and sinE) involved in the biosynthesis of lipolanthine was identified in the genome of an actinobacterium Sinosporangium siamense. Heterologous coexpression of a precursor peptide coding gene sinA and lanthipeptide synthetase coding gene sinKC in the host Escherichia coli strain BL21(DE3) resulted in the synthesis of a new lanthipeptide, sinosporapeptin. It contained unusual amino acids, including one labionin and two dehydrobutyrine residues, as determined using NMR and MS analyses. Another coexpression experiment with two additional genes of decarboxylase (sinD) and N-acetyl transferase (sinE) resulted in the production of a lipolanthine-like modified sinosporapeptin.

Keywords: evolution in vitro;genetic fixation;genetic polymorphism;mutation;varicella vaccine

Figure & Data

References

Citations

Citations to this article as recorded by

Heterologous biosynthesis of myxobacterial lanthipeptides melittapeptins
Issara Kaweewan, Keiichiro Mukai, Pratchaya Rukthanapitak, Hiroyuki Nakagawa, Takeshi Hosaka, Shinya Kodani
Applied Microbiology and Biotechnology.2024;[Epub] CrossRef
Facile Method for Determining Lanthipeptide Stereochemistry
Youran Luo, Shuyun Xu, Autumn M. Frerk, Wilfred A. van der Donk
Analytical Chemistry.2024; 96(4): 1767. CrossRef
Antimicrobial Peptides Derived from Bacteria: Classification, Sources, and Mechanism of Action against Multidrug-Resistant Bacteria
Raynichka Mihaylova-Garnizova, Slavena Davidova, Yordan Hodzhev, Galina Satchanska
International Journal of Molecular Sciences.2024; 25(19): 10788. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Heterologous Production and Structure Determination of a New Lanthipeptide Sinosporapeptin Using a Cryptic Gene Cluster in an Actinobacterium Sinosporangium siamense

J. Microbiol. 2023;61(6):641-648. Published online June 12, 2023

DOI: https://doi.org/10.1007/s12275-023-00059-z

Related articles