Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children

Nai-Hua Liu; Hong-Qian Liu; Jia-Yi Zheng; Meng-Lu Zhu; Li-Hao Wu; Hua-Feng Pan; Xing-Xiang He

doi:10.1007/s12275-023-00069-x

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Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children: Nai-Hua Liu ^1,2, Hong-Qian Liu ³, Jia-Yi Zheng ⁴, Meng-Lu Zhu ¹, Li-Hao Wu ³, Hua-Feng Pan ¹, Xing-Xiang He ^2,3; Journal of Microbiology 2023;61(8):741-753.
DOI: https://doi.org/10.1007/s12275-023-00069-x
Published online: September 4, 2023

¹Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, People’s Republic of China, ²Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Nonglin Down Street 19, Guangzhou 510080, People’s Republic of China, ³Department of Gastroenterology, Research Center for Engineering Techniques of Microbiota‑Targeted Therapies of Guangdong Province, The First Affiliated Hospital of Guangdong Pharmaceutical University, Nonglin Down Street 19, Guangzhou 510080, People’s Republic of China, ⁴Department of Neurology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, People’s Republic of China

Corresponding author: Hua-Feng Pan ,
Xing-Xiang He ,

Received: 21 May 2023 • Revised: 25 July 2023 • Accepted: 30 July 2023

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Abstract

Accumulating studies have raised concerns about gut dysbiosis associating autism spectrum disorder (ASD) and its related symptoms. However, the effect of gut microbiota modification on the Chinese ASD population and its underlying mechanism were still elusive. Herein, we enrolled 24 ASD children to perform the first course of fresh washed microbiota transplantation (WMT), 18 patients decided to participate the second course, 13 of which stayed to participate the third course, and there were 8 patients at the fourth course. Then we evaluated the effects of fresh WMT on these patients and their related symptoms. Our results found that the sleeping disorder symptom was positively interrelated to ASD, fresh WMT significantly alleviated ASD and its sleeping disorder and constipation symptoms. In addition, WMT stably and continuously downregulated Bacteroides/ Flavonifractor/Parasutterella while upregulated Prevotella_9 to decrease toxic metabolic production and improve detoxification by regulating glycolysis/myo-inositol/D-glucuronide/D-glucarate degradation, L-1,2-propanediol degradation, fatty acid β-oxidation. Thus, our results suggested that fresh WMT moderated gut microbiome to improve the behavioral and sleeping disorder symptoms of ASD via decrease toxic metabolic production and improve detoxification. Which thus provides a promising gut ecological strategy for ASD children and its related symptoms treatments.

Keywords: Autism spectrum disorder · Washed microbiota transplantation · Sleeping disorder · Detoxification

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Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children

J. Microbiol. 2023;61(8):741-753. Published online September 4, 2023

DOI: https://doi.org/10.1007/s12275-023-00069-x

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