Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum

Hyunwoo Shin; Yeongjin Baek; Dukwon Lee; Yongbin Xu; Yonghoon Kwon; Inseong Jo; Nam-Chul Ha

doi:10.1007/s12275-023-00095-9

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Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum: Hyunwoo Shin ¹, Yeongjin Baek ¹, Dukwon Lee ¹, Yongbin Xu ², Yonghoon Kwon ¹, Inseong Jo ³, Nam-Chul Ha ^1,4; Journal of Microbiology 2023;61(12):1033-1041.
DOI: https://doi.org/10.1007/s12275-023-00095-9
Published online: December 20, 2023

¹Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, CALS, Seoul National University, Seoul 08826, Republic of Korea, ²Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, People’s Republic of China, ³Infectious Diseases Therapeutic Research Center, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea, ⁴Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea¹Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, CALS, Seoul National University, Seoul 08826, Republic of Korea, ²Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, People’s Republic of China, ³Infectious Diseases Therapeutic Research Center, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea, ⁴Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea

Corresponding author: Inseong Jo ,
Nam-Chul Ha ,

Received: 4 October 2023 • Revised: 7 November 2023 • Accepted: 7 November 2023

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Abstract

Escherichia coli RclA and Staphylococcus aureus MerA are part of the Group I flavoprotein disulfide reductase (FDR) family and have been implicated in the contribution to bacterial pathogenesis by defending against the host immune response. Fusobacterium nucleatum is a pathogenic, anaerobic Gram-negative bacterial species commonly found in the human oral cavity and gastrointestinal tract. In this study, we discovered that the F. nucleatum protein FN0820, belonging to the Group I FDR family, exhibited a higher activity of a Cu2+- dependent NADH oxidase than E. coli RclA. Moreover, FN0820 decreased the dissolved oxygen level in the solution with higher NADH oxidase activity. We found that L-tryptophan and its analog 5-hydroxytryptophan inhibit the FN0820 activities of NADH oxidase and the concomitant reduction of oxygen. Our results have implications for developing new treatment strategies against pathogens that defend the host immune response with Group I FDRs.

Keywords: Parahaliea maris;genomic analysis;novel species;polyphasic taxonomy

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Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum

J. Microbiol. 2023;61(12):1033-1041. Published online December 20, 2023

DOI: https://doi.org/10.1007/s12275-023-00095-9

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